E2F1
GeneName
E2F1
Summary
E2F1, also known as transcription factor E2F1 or E2F-1, is a 47kDa transcription factor that plays a pivotal role in regulating the cell cycle and gene expression. It is primarily located in the nucleus, where it binds to specific DNA sequences to activate or repress the transcription of target genes involved in cell proliferation and apoptosis. E2F1 is part of the Rb-E2F complex and interacts with RNA polymerase II to facilitate DNA-templated transcription. It is involved in various biological processes, including cellular responses to stress signals, DNA damage checkpoint signalling, and developmental processes such as forebrain development and spermatogenesis.
Importance
E2F1 is relevant to: - Cancer research due to its role in cell cycle regulation and its association with tumourigenesis when dysregulated - Apoptosis and cellular responses to DNA damage, making it a target for therapeutic interventions in diseases characterised by abnormal cell survival - Metabolic regulation as it responds to fatty acids and influences fat cell differentiation and proliferation - Neurobiology, particularly in the context of nerve growth factor signalling and glial cell proliferation
Top Products
For researchers investigating E2F1, we recommend two excellent primary antibodies that cater to a variety of applications. The first is the well-cited polyclonal antibody, Anti-E2F1 antibody (ab137415), which has garnered 10 citations and is particularly effective in Western blotting (WB) and immunocytochemistry (ICC). This product is a trusted choice for those looking to study E2F1 in detail. Additionally, we offer the recombinant antibody, Anti-E2F1 antibody [1D12] (ab288369), which has received 13 citations and is validated for a broader range of applications, including WB, immunohistochemistry (IHC), ICC, and flow cytometry (FC). This recombinant option ensures batch-to-batch consistency, making it an ideal selection for researchers seeking reliable and versatile E2F1 detection.
Abcam Product Citation Summary
The data indicates that the E2F1 target is being investigated in the context of TDP-43 proteinopathies, a condition associated with neurodegenerative diseases. The use of Western blotting in mouse models suggests a focus on understanding the molecular mechanisms underlying this pathology.
Abcam Product Citation Table
Function
Transcription activator that binds DNA cooperatively with DP proteins through the E2 recognition site, 5'-TTTC[CG]CGC-3' found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication (PubMed:10675335, PubMed:12717439, PubMed:17050006, PubMed:17704056, PubMed:18625225, PubMed:28992046). The DRTF1/E2F complex functions in the control of cell-cycle progression from G1 to S phase (PubMed:10675335, PubMed:12717439, PubMed:17704056). E2F1 binds preferentially RB1 in a cell-cycle dependent manner (PubMed:10675335, PubMed:12717439, PubMed:17704056). It can mediate both cell proliferation and TP53/p53-dependent apoptosis (PubMed:8170954). Blocks adipocyte differentiation by binding to specific promoters repressing CEBPA binding to its target gene promoters (PubMed:20176812). Directly activates transcription of PEG10 (PubMed:17050006, PubMed:18625225, PubMed:28992046). Positively regulates transcription of RRP1B (PubMed:20040599).
Post-translational modifications
Phosphorylated by CDK2 and cyclin A-CDK2 in the S-phase (PubMed:12717439, PubMed:7838523). Phosphorylation at Ser-364 by CHEK2 stabilizes E2F1 upon DNA damage and regulates its effect on transcription and apoptosis (PubMed:12717439). Phosphorylation at Ser-403 by GSK3B promotes interaction with USP11, leading to its deubiquitination and stabilization (PubMed:28992046).
Ubiquitinated via 'Lys-63'-linked ubiquitin, leading to its degradation (PubMed:28992046). Deubiquitinated by USP11 following phosphorylation by GSK3B, promoting its stability (PubMed:28992046).
Acetylation stimulates DNA-binding. Enhanced under stress conditions such as DNA damage and inhibited by retinoblastoma protein RB1. Regulated by KAP1/TRIM28 which recruits HDAC1 to E2F1 resulting in deacetylation. Acetylated by P/CAF/KAT2B.
Methylation at Lys-185 by SETD7 promotes E2F1 ubiquitin-dependent proteasomal degradation.
Sequence Similarities
Belongs to the E2F/DP family.
Cellular localization
- Nucleus
Alternative names
RBBP3, E2F1, Transcription factor E2F1, E2F-1, PBR3, Retinoblastoma-associated protein 1, Retinoblastoma-binding protein 3, pRB-binding protein E2F-1, RBAP-1, RBBP-3
Database links
swissprot:Q01094 entrezGene:1869 omim:189971
Other research areas
- Oncology