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EGF

GeneName

EGF

Summary

EGF, also known as hEGF or pro epidermal growth factor, is a 134 kDa growth factor that plays a vital role in various biological processes. It is primarily expressed in the extracellular region and is associated with cellular components such as the plasma membrane and extracellular exosomes. EGF binds to the epidermal growth factor receptor (EGFR) and activates several signalling pathways, including the ERK1 and ERK2 cascade, which are crucial for cell proliferation, migration, and differentiation. Its functions extend to angiogenesis, epithelial cell proliferation, and the development of mammary gland alveoli, highlighting its importance in tissue growth and repair.

Importance

EGF is relevant to: - Cancer research, particularly in understanding tumour growth and metastasis due to its role in promoting cell proliferation and migration. - Tissue engineering and regenerative medicine, as it is involved in wound healing and tissue repair processes. - Developmental biology, given its critical function in embryonic development and organogenesis. - Cardiovascular research, as it plays a role in angiogenesis and endothelial cell function.

Top Products

For researchers investigating EGF, we recommend two excellent primary antibodies. The first is the well-cited polyclonal antibody, Anti-EGF antibody (ab9695), which has garnered 35 citations, reflecting its reliability in the field. This antibody is particularly effective for Western blotting (WB) and ELISA applications. Additionally, we offer the recombinant antibody, Anti-EGF antibody [EPR19899] (ab206423), which is validated for use in Western blotting (WB). This recombinant option provides the advantage of batch-to-batch consistency, making it a dependable choice for your research needs. The Human EGF Matched Antibody Pair Kit (ab218186) is an excellent option for researchers looking to study EGF with precision and reliability.

Abcam Product Citation Summary

The data indicates that EGF antibodies from Abcam have been primarily used in studies involving mouse models, particularly focusing on muscle stem cell proliferation. Additionally, EGF measurement has been conducted in human cell cultures, highlighting its relevance in cancer research.

Abcam Product Citation Table

Product Code
Species
Application
Study Context
PMID
ab206423
Mouse
WB
MuSC proliferation
32839552
ab217772
Nude mouse
Study involving nude mice
32126150
ab217772
Human
Culture medium of LN-299, LN-18, and A172 cells
32126150

Function

EGF stimulates the growth of various epidermal and epithelial tissues in vivo and in vitro and of some fibroblasts in cell culture. Magnesiotropic hormone that stimulates magnesium reabsorption in the renal distal convoluted tubule via engagement of EGFR and activation of the magnesium channel TRPM6. Can induce neurite outgrowth in motoneurons of the pond snail Lymnaea stagnalis in vitro (PubMed:10964941).

Involvement in disease

Hypomagnesemia 4

HOMG4

A disorder characterized by massive renal hypomagnesemia and normal levels of serum calcium and calcium excretion. Clinical features include seizures, mild-to moderate psychomotor retardation, and brisk tendon reflexes.

None

The disease is caused by variants affecting the gene represented in this entry.

Post-translational modifications

O-glycosylated with core 1-like and core 2-like glycans. It is uncertain if Ser-954 or Thr-955 is O-glycosylated. The modification here shows glycan heterogeneity: HexHexNAc (major) and Hex2HexNAc2 (minor).

Tissue Specificity

Expressed in kidney, salivary gland, cerebrum and prostate.

Cellular localization

Alternative names

Pro-epidermal growth factor, EGF

swissprot:P01133 omim:131530 entrezGene:1950

Other research areas