ELANE
GeneName
ELANE
Summary
ELANE, also known as neutrophil elastase or HLE, is a 29 kDa serine protease primarily expressed in neutrophils. It is localised in various cellular compartments, including azurophil granules, phagocytic vesicles, and the extracellular space. ELANE plays a pivotal role in the immune response by mediating the degradation of extracellular matrix components and facilitating neutrophil migration during inflammation. Its enzymatic activity includes endopeptidase and protease functions, which are essential for the defence against bacterial infections, particularly Gram-negative bacteria. Additionally, ELANE is involved in the regulation of cytokine production and has implications in various inflammatory processes.
Importance
ELANE is relevant to: - The innate immune response, particularly in the context of neutrophil function and inflammation. - The development of therapeutic strategies targeting inflammatory diseases, given its role in regulating inflammatory mediators. - Understanding the mechanisms of tissue remodelling and repair, as it contributes to extracellular matrix disassembly. - The pathogenesis of conditions associated with dysregulated neutrophil activity, such as chronic obstructive pulmonary disease (COPD) and cystic fibrosis.
Top Products
For researchers investigating ELANE, we recommend two excellent primary antibodies. The first is the well-cited polyclonal antibody, Anti-Neutrophil Elastase antibody (ab68672), which has garnered 268 citations, highlighting its reliability in immunohistochemistry (IHC). Additionally, we offer the recombinant antibody, Anti-Neutrophil Elastase antibody [EPR7479] (ab131260). This versatile monoclonal antibody has been validated for use in IHC, immunocytochemistry (ICC), western blotting (WB), and flow cytometry (FC), making it an ideal choice for those seeking consistent performance across multiple applications. With 48 citations, it is also gaining traction in the research community. The Human Neutrophil Elastase ELISA Kit (ab270204), supported by 8 citations, is an excellent option for researchers looking to accurately measure ELANE levels in their samples.
Abcam Product Citation Summary
The data indicates that ELANE is being studied in various contexts, including malignancies, lung inflammation, and acute kidney injury. The use of antibodies in both mouse and human samples highlights the relevance of ELANE in different biological systems, particularly in immune responses and cancer pathology.
Abcam Product Citation Table
Function
Serine protease that modifies the functions of natural killer cells, monocytes and granulocytes. Inhibits C5a-dependent neutrophil enzyme release and chemotaxis (PubMed:15140022). Promotes cleavage of GSDMB, thereby inhibiting pyroptosis (PubMed:36899106). Promotes blood coagulation (PubMed:20676107). Through the activation of the platelet fibrinogen receptor integrin alpha-IIb/beta-3, potentiates platelet aggregation induced by a threshold concentration of cathepsin G (CTSG) (PubMed:25211214, PubMed:9111081). Cleaves and thus inactivates tissue factor pathway inhibitor (TFPI) (PubMed:20676107, PubMed:25211214). Capable of killing E.coli but not S.aureus in vitro; digests outer membrane protein A (ompA) in E.coli and K.pneumoniae (PubMed:10947984).
Involvement in disease
Cyclic haematopoiesis
CH
Autosomal dominant disease in which blood-cell production from the bone marrow oscillates with 21-day periodicity. Circulating neutrophils vary between almost normal numbers and zero. During intervals of neutropenia, affected individuals are at risk for opportunistic infection. Monocytes, platelets, lymphocytes and reticulocytes also cycle with the same frequency.
None
The disease is caused by variants affecting the gene represented in this entry.
Neutropenia, severe congenital 1, autosomal dominant
SCN1
A disorder of hematopoiesis characterized by maturation arrest of granulopoiesis at the level of promyelocytes with peripheral blood absolute neutrophil counts below 0.5 x 10(9)/l and early onset of severe bacterial infections.
None
The disease is caused by variants affecting the gene represented in this entry.
Sequence Similarities
Belongs to the peptidase S1 family. Elastase subfamily.
Tissue Specificity
Bone marrow cells. Neutrophil (PubMed:10947984).
Cellular localization
- Cytoplasmic vesicle
- Phagosome
- Localized in phagolysosomes following ingestion of E.coli by neutrophils.
Alternative names
ELA2, ELANE, Neutrophil elastase, Bone marrow serine protease, Elastase-2, Human leukocyte elastase, Medullasin, PMN elastase, HLE