ELAVL1
GeneName
ELAVL1
Summary
ELAVL1, also known as HuR, is a 36 kDa RNA-binding protein predominantly expressed in the cytoplasm and nucleus of various cell types. It plays a crucial role in post-transcriptional regulation of gene expression by binding to AU-rich elements in the 3'-UTR of mRNAs, thus stabilising them and influencing their translation. ELAVL1 is involved in several cellular processes, including mRNA stabilization and destabilization, and has been implicated in stress granule formation. It is localised in multiple subcellular compartments such as the cytoplasm, nucleus, and P-bodies, and is important for maintaining cellular responses to stress and regulating gene expression in various contexts.
Importance
ELAVL1 is relevant to: - Cancer biology, as it regulates mRNA stability and translation of oncogenes, influencing tumour progression and response to therapy - Neurodegenerative diseases, where it may affect the stability of mRNAs involved in neuronal function and survival - Immune responses, due to its role in modulating the expression of cytokines and other immune-related genes - Metabolic regulation, as it is involved in the response to glucose and may influence metabolic pathways.
Top Products
For researchers investigating ELAVL1, we highly recommend the top-selling recombinant antibody, Anti-HuR / ELAVL1 antibody [EPR17397] (ab200342). This antibody has been validated in knockout models, ensuring its reliability for your experiments. It is suitable for a variety of applications, including Western blotting (WB), immunocytochemistry (ICC), immunohistochemistry (IHC), immunoprecipitation (IP), and flow cytometry (FC). With 85 citations, this antibody is well-regarded in the research community, making it an excellent choice for those studying ELAVL1.
Abcam Product Citation Summary
The ELAVL1 antibody (ab200342) has been extensively used in various studies involving human cells, particularly in the context of asthma, PDGF treatment effects, and TGF-β1 expression. The applications primarily include Western blotting and immunocytochemistry, indicating its relevance in protein detection and analysis in human ASM and NSCLC cells.
Abcam Product Citation Table
Domain
The first RRM (RNA recognition motif) domain is essential for binding to AU-rich elements.
Function
RNA-binding protein that binds to the 3'-UTR region of mRNAs and increases their stability (PubMed:14517288, PubMed:18285462, PubMed:31358969). Involved in embryonic stem cell (ESC) differentiation: preferentially binds mRNAs that are not methylated by N6-methyladenosine (m6A), stabilizing them, promoting ESC differentiation (By similarity). Has also been shown to be capable of binding to m6A-containing mRNAs and contributes to MYC stability by binding to m6A-containing MYC mRNAs (PubMed:32245947). Binds to poly-U elements and AU-rich elements (AREs) in the 3'-UTR of target mRNAs (PubMed:14731398, PubMed:17632515, PubMed:18285462, PubMed:23519412, PubMed:8626503). Binds avidly to the AU-rich element in FOS and IL3/interleukin-3 mRNAs. In the case of the FOS AU-rich element, binds to a core element of 27 nucleotides that contain AUUUA, AUUUUA, and AUUUUUA motifs. Binds preferentially to the 5'-UUUU[AG]UUU-3' motif in vitro (PubMed:8626503). With ZNF385A, binds the 3'-UTR of p53/TP53 mRNA to control their nuclear export induced by CDKN2A. Hence, may regulate p53/TP53 expression and mediate in part the CDKN2A anti-proliferative activity. May also bind with ZNF385A the CCNB1 mRNA (By similarity). Increases the stability of the leptin mRNA harboring an AU-rich element (ARE) in its 3' UTR (PubMed:29180010).
Post-translational modifications
Phosphorylated by MAPKAPK2 (PubMed:14517288). Phosphorylated by PRKCD (PubMed:18285462).
Methylated at Arg-217 by CARM1 in macrophages in response to LPS challenge.
Sequence Similarities
Belongs to the RRM elav family.
Tissue Specificity
Ubiquitous. Detected in brain, liver, thymus and muscle.
Cellular localization
- Cytoplasm
- Nucleus
- Cytoplasm
- Stress granule
- Cytoplasm
- P-body
- Translocates into the cytoplasm following phosphorylation by MAPKAPK2 (PubMed:14517288). Likewise, phosphorylation by PRKCD promotes translocation from the nucleus into the cytoplasm, where it is associated with free and cytoskeleton-bound polysomes (PubMed:18285462). Localizes to the stress granules in the presence of PLEKHN1 (By similarity).
Alternative names
HUR, ELAVL1, ELAV-like protein 1, Hu-antigen R, HuR