ENAH

Domain

The EVH2 domain is comprised of 3 regions. Block A is a thymosin-like domain required for G-actin binding. The KLKR motif within this block is essential for the G-actin binding and for actin polymerization. Block B is required for F-actin binding and subcellular location, and Block C for tetramerization.

Function

Ena/VASP proteins are actin-associated proteins involved in a range of processes dependent on cytoskeleton remodeling and cell polarity such as axon guidance and lamellipodial and filopodial dynamics in migrating cells. ENAH induces the formation of F-actin rich outgrowths in fibroblasts. Acts synergistically with BAIAP2-alpha and downstream of NTN1 to promote filipodia formation (By similarity).

Post-translational modifications

NTN1-induced PKA phosphorylation on Ser-265 directly parallels the formation of filopodial protrusions.

Sequence Similarities

Belongs to the Ena/VASP family.

Tissue Specificity

Expressed in myoepithelia of parotid, breast, bronchial glands and sweat glands. Expressed in colon-rectum muscolaris mucosae epithelium, pancreas acinar ductal epithelium, endometrium epithelium, prostate fibromuscolar stroma and placenta vascular media. Overexpressed in a majority of breast cancer cell lines and primary breast tumor lesions.

Cellular localization

• Cytoplasm
• Cytoplasm
• Cytoskeleton
• Cell projection
• Lamellipodium
• Cell projection
• Filopodium
• Synapse
• Cell junction
• Focal adhesion
• Targeted to the leading edge of lamellipodia and filopodia by MRL family members. Colocalizes at filopodial tips with a number of other proteins including vinculin and zyxlin. Colocalizes with N-WASP at the leading edge. Colocalizes with GPHN and PFN at synapses (By similarity).

Alternative names

MENA, ENAH, Protein enabled homolog

Database links

swissprot:Q8N8S7 entrezGene:513095 entrezGene:100720009 entrezGene:55740 omim:609061