ENO1
GeneName
ENO1
Summary
ENO1, also known as enolase, alpha enolase, or MBP-1, is a 47 kDa glycolytic enzyme that plays a crucial role in the conversion of 2-phosphoglycerate to phosphoenolpyruvate in the glycolytic pathway. It is expressed in various tissues, including muscle and brain, and is localised in multiple cellular compartments such as the cytoplasm, nucleus, and plasma membrane. ENO1 also exhibits additional functions beyond its enzymatic role, including DNA-binding transcription repressor activity, and involvement in muscle contraction regulation and response to viral infections. Its diverse functions are mediated through interactions with various proteins and nucleic acids, highlighting its importance in both metabolic and regulatory processes.
Importance
ENO1 is relevant to: - Cancer research, as it is implicated in the regulation of cell growth and apoptosis, making it a potential target for therapeutic intervention. - Metabolic disorders, due to its central role in glycolysis and gluconeogenesis, influencing energy production and utilisation. - Muscle physiology, given its involvement in muscle contraction and regulation of ATP biosynthesis. - Viral pathogenesis, as it plays a role in the cellular response to viral infections, potentially impacting viral replication and host defence mechanisms.
Top Products
For researchers investigating ENO1, we highly recommend the top-selling recombinant antibody, Anti-ENO1 + ENO2 + ENO3 antibody [EPR10863(B)] (ab155102). This antibody has been validated for a variety of applications, including Western blotting (WB), immunocytochemistry (ICC), immunoprecipitation (IP), and flow cytometry (FC), making it a versatile tool for your research needs. With 41 citations, it is well-regarded in the scientific community, reflecting its reliability and effectiveness in detecting ENO1 and its related isoforms. This product is an excellent choice for those seeking consistent performance in their experiments. The ENO1 Assay Kit (alpha Enolase, Human) (ab117994), supported by 8 citations, is an excellent option for researchers looking to accurately measure ENO1 levels in their samples.
Abcam Product Citation Summary
The data indicates that ENO1 is being studied in the context of human melanoma, specifically in A375 cells. This suggests a focus on the role of ENO1 in cancer biology.
Abcam Product Citation Table
Developmental stage
During ontogenesis, there is a transition from the alpha/alpha homodimer to the alpha/beta heterodimer in striated muscle cells, and to the alpha/gamma heterodimer in nerve cells.
Function
Glycolytic enzyme the catalyzes the conversion of 2-phosphoglycerate to phosphoenolpyruvate (PubMed:1369209, PubMed:29775581). In addition to glycolysis, involved in various processes such as growth control, hypoxia tolerance and allergic responses (PubMed:10802057, PubMed:12666133, PubMed:2005901, PubMed:29775581). May also function in the intravascular and pericellular fibrinolytic system due to its ability to serve as a receptor and activator of plasminogen on the cell surface of several cell-types such as leukocytes and neurons (PubMed:12666133). Stimulates immunoglobulin production (PubMed:1369209).
Isoform MBP-1
Binds to the myc promoter and acts as a transcriptional repressor. May be a tumor suppressor.
Pathway
Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 4/5.
Post-translational modifications
ISGylated.
Lysine 2-hydroxyisobutyrylation (Khib) by p300/EP300 activates the phosphopyruvate hydratase activity.
Sequence Similarities
Belongs to the enolase family.
Tissue Specificity
The alpha/alpha homodimer is expressed in embryo and in most adult tissues. The alpha/beta heterodimer and the beta/beta homodimer are found in striated muscle, and the alpha/gamma heterodimer and the gamma/gamma homodimer in neurons.
Cellular localization
- Cytoplasm
- Cell membrane
- Cytoplasm
- Myofibril
- Sarcomere
- M line
- Can translocate to the plasma membrane in either the homodimeric (alpha/alpha) or heterodimeric (alpha/gamma) form. ENO1 is localized to the M line.
- Isoform MBP-1
- Nucleus
Alternative names
ENO1L1, MBPB1, MPB1, ENO1, Alpha-enolase, 2-phospho-D-glycerate hydro-lyase, C-myc promoter-binding protein, Enolase 1, MBP-1, MPB-1, Non-neural enolase, Phosphopyruvate hydratase, Plasminogen-binding protein, NNE
Database links
swissprot:P06733 swissprot:P13929 entrezGene:2027 entrezGene:2026 entrezGene:2023 swissprot:P09104 omim:172430 omim:131370 omim:131360