FASN
GeneName
FASN
Summary
FASN, also known as fatty acid synthase or Fas, is a multi-functional enzyme complex with a molecular weight of 273 kDa that plays a central role in fatty acid biosynthesis. It is primarily located in the cytoplasm and is involved in the synthesis of long-chain fatty acids from acetyl-CoA and malonyl-CoA through a series of enzymatic reactions. FASN exhibits various activities including acyl-carrier-protein binding, and its functions extend to the modulation of cellular responses to interleukin-4 and the establishment of the endothelial intestinal barrier. This enzyme is also implicated in processes such as mammary gland development and inflammatory responses, highlighting its importance in metabolic regulation and cellular differentiation.
Importance
FASN is relevant to: - Metabolic disorders, including obesity and diabetes, due to its role in fatty acid metabolism and energy homeostasis. - Cancer research, as it is often overexpressed in various cancers, contributing to the altered lipid metabolism in tumour cells. - Inflammatory diseases, since it is involved in the inflammatory response and differentiation of immune cells such as monocytes and neutrophils. - Cardiovascular health, given its influence on lipid profiles and vascular function through fatty acid synthesis.
Top Products
For researchers investigating FASN, we highly recommend the top-selling recombinant antibody, Anti-Fatty Acid Synthase antibody [EPR7466] (ab128870). This well-cited antibody has garnered 93 citations, reflecting its reliability and trust within the scientific community. It has been validated in knockout models and is suitable for a variety of applications, including Western blotting (WB), immunohistochemistry (IHC), immunocytochemistry (ICC), immunoprecipitation (IP), and flow cytometry (FC). This versatility makes it an excellent choice for those seeking consistent and effective detection of FASN in their experiments. The Human FAS ELISA Kit (ab183360) is an excellent option for researchers looking to measure FAS levels in their samples.
Abcam Product Citation Summary
The data indicates that FASN is being studied in various contexts related to cancer, lipid metabolism, and apoptosis. The use of Abcam antibodies in different species, particularly in mouse and human tissues, highlights the relevance of FASN in metabolic processes and disease states such as colon cancer and liver conditions.
Abcam Product Citation Table
Function
Fatty acid synthetase is a multifunctional enzyme that catalyzes the de novo biosynthesis of long-chain saturated fatty acids starting from acetyl-CoA and malonyl-CoA in the presence of NADPH. This multifunctional protein contains 7 catalytic activities and a site for the binding of the prosthetic group 4'-phosphopantetheine of the acyl carrier protein ([ACP]) domain.
(Microbial infection) Fatty acid synthetase activity is required for SARS coronavirus-2/SARS-CoV-2 replication.
Pathway
Lipid metabolism; fatty acid biosynthesis.
Post-translational modifications
S-nitrosylation of Fatty acid synthase at cysteine residues Cys-1471 or Cys-2091 is important for the enzyme dimerization. In adipocytes, S-nitrosylation of Fatty acid synthase occurs under physiological conditions and gradually increases during adipogenesis.
Tissue Specificity
Ubiquitous. Prominent expression in brain, lung, liver and mammary gland.
Cellular localization
- Cytoplasm
- Melanosome
- Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
Alternative names
FAS, FASN, Fatty acid synthase, Type I fatty acid synthase