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Domain

The FERM domain is not correctly detected by PROSITE or Pfam techniques because it contains the insertion of a PH domain.

The PH domain binds phospholipids. Binds preferentially phosphatidylinositol-3,4,5-trisphosphate, and has lower affinity for phosphatidylinositol-4,5-bisphosphate (PubMed:22030399).

The N-terminal region displays a ubiquitin-type fold and mediates interaction with membranes containing negatively charged phosphatidylinositol phosphate via a surface enriched in positively charged residues.

Function

Scaffolding protein that enhances integrin activation mediated by TLN1 and/or TLN2, but activates integrins only weakly by itself. Binds to membranes enriched in phosphoinositides. Enhances integrin-mediated cell adhesion onto the extracellular matrix and cell spreading; this requires both its ability to interact with integrins and with phospholipid membranes. Required for the assembly of focal adhesions. Participates in the connection between extracellular matrix adhesion sites and the actin cytoskeleton and also in the orchestration of actin assembly and cell shape modulation. Recruits FBLIM1 to focal adhesions. Plays a role in the TGFB1 and integrin signaling pathways. Stabilizes active CTNNB1 and plays a role in the regulation of transcription mediated by CTNNB1 and TCF7L2/TCF4 and in Wnt signaling.

Sequence similarities

Belongs to the kindlin family.

Tissue specificity

Ubiquitous. Found in numerous tumor tissues.

Cellular localization

  • Cytoplasm
  • Cytoplasm
  • Cell cortex
  • Cytoplasm
  • Cytoskeleton
  • Cytoplasm
  • Cytoskeleton
  • Stress fiber
  • Cell junction
  • Focal adhesion
  • Membrane
  • Peripheral membrane protein
  • Cytoplasmic side
  • Cell projection
  • Lamellipodium membrane
  • Peripheral membrane protein
  • Cytoplasmic side
  • Nucleus
  • Cytoplasm
  • Myofibril
  • Sarcomere
  • I band
  • Cell surface
  • Colocalizes with actin stress fibers at cell-ECM focal adhesion sites. Colocalizes with ITGB3 at lamellipodia at the leading edge of spreading cells. Binds to membranes that contain phosphatidylinositides.

Alternative names

KIND2, MIG2, PLEKHC1, FERMT2, Fermitin family homolog 2, Kindlin-2, Mitogen-inducible gene 2 protein, Pleckstrin homology domain-containing family C member 1, MIG-2, PH domain-containing family C member 1

Target type

Proteins

Primary research area

Oncology

Molecular weight

77861Da

We found 6 products in 2 categories

Primary Antibodies

Reactive species

Cell Lines & Lysates

Cell type

Species or organism