Skip to main content

FOXP3_HUMAN

Domain

The fork-head DNA-binding domain is essential for its dimerization and interaction with NFATC2.

Function

Transcriptional regulator which is crucial for the development and inhibitory function of regulatory T-cells (Treg) (PubMed:17377532, PubMed:21458306, PubMed:23947341, PubMed:24354325, PubMed:24722479, PubMed:24835996, PubMed:30513302, PubMed:32644293). Plays an essential role in maintaining homeostasis of the immune system by allowing the acquisition of full suppressive function and stability of the Treg lineage, and by directly modulating the expansion and function of conventional T-cells (PubMed:23169781). Can act either as a transcriptional repressor or a transcriptional activator depending on its interactions with other transcription factors, histone acetylases and deacetylases (PubMed:17377532, PubMed:21458306, PubMed:23947341, PubMed:24354325, PubMed:24722479). The suppressive activity of Treg involves the coordinate activation of many genes, including CTLA4 and TNFRSF18 by FOXP3 along with repression of genes encoding cytokines such as interleukin-2 (IL2) and interferon-gamma (IFNG) (PubMed:17377532, PubMed:21458306, PubMed:23947341, PubMed:24354325, PubMed:24722479). Inhibits cytokine production and T-cell effector function by repressing the activity of two key transcription factors, RELA and NFATC2 (PubMed:15790681). Mediates transcriptional repression of IL2 via its association with histone acetylase KAT5 and histone deacetylase HDAC7 (PubMed:17360565). Can activate the expression of TNFRSF18, IL2RA and CTLA4 and repress the expression of IL2 and IFNG via its association with transcription factor RUNX1 (PubMed:17377532). Inhibits the differentiation of IL17 producing helper T-cells (Th17) by antagonizing RORC function, leading to down-regulation of IL17 expression, favoring Treg development (PubMed:18368049). Inhibits the transcriptional activator activity of RORA (PubMed:18354202). Can repress the expression of IL2 and IFNG via its association with transcription factor IKZF4 (By similarity).

Involvement in disease

Immunodeficiency polyendocrinopathy, enteropathy, X-linked syndrome

IPEX

Characterized by neonatal onset insulin-dependent diabetes mellitus, infections, secretory diarrhea, thrombocytopenia, anemia and eczema. It is usually lethal in infancy.

None

The disease is caused by variants affecting the gene represented in this entry.

Post-translational modifications

Polyubiquitinated, leading to its proteasomal degradation in regulatory T-cells (Treg) which is mediated by STUB1 in a HSPA1A/B-dependent manner. Deubiquitinated by USP7 and USP44; leading to increase in protein stability.

Phosphorylation at Ser-418 regulates its transcriptional repressor activity and consequently, regulatory T-cells (Treg) suppressive function. Dephosphorylated at Ser-418 by protein phosphatase 1 (PP1) in Treg cells derived from patients with rheumatoid arthritis. Phosphorylation by CDK2 negatively regulates its transcriptional activity and protein stability (By similarity).

Acetylation on lysine residues stabilizes FOXP3 and promotes differentiation of T-cells into induced regulatory T-cells (iTregs) associated with suppressive functions (PubMed:17360565, PubMed:24835996). Acetylation is mediated by a coordinated action of KAT5 and EP300/p300 acetyltransferases: EP300/p300 is required to enhance KAT5 autoacetylation, promoting acetylation of FOXP3 by KAT5 (PubMed:24835996). Deacetylated by SIRT1 (PubMed:22312127).

Undergoes proteolytic cleavage in activated regulatory T-cells (Treg), and can be cleaved at either the N- or C-terminal site, or at both sites.

Cellular localization

  • Nucleus
  • Cytoplasm
  • Predominantly expressed in the cytoplasm in activated conventional T-cells whereas predominantly expressed in the nucleus in regulatory T-cells (Treg). The 41 kDa form derived by proteolytic processing is found exclusively in the chromatin fraction of activated Treg cells (By similarity).

Alternative names

  • IPEX
  • JM2
  • JM2
  • FOXP3
  • IPEX
  • Forkhead box protein P3
  • Scurfin

Target type

Proteins

Primary research area

Immuno-oncology

Other research areas

  • Immunology & Infectious Disease

Molecular weight

47244Da

We found 39 products in 5 categories

Primary Antibodies

Assay Kits

Target

Reactive species

Detection method

Proteins & Peptides

Target

Species of origin

Nature

Multiplex Kits

Cell Lines & Lysates

Target

Cell type

Species or organism

Search our catalogue for 'FOXP3_HUMAN' (39)

Products

ab20034

Anti-FOXP3 antibody [236A/E7]

20ul selling size
Advanced Validation
Recombinant

ab215206

Anti-FOXP3 antibody [EPR22102-37]

20ul selling size
RabMAb
Advanced Validation
Recombinant

ab309108

Anti-FOXP3 antibody [mAbcam 450]

20ul selling size
Recombinant

ab253297

Anti-FOXP3 antibody [221D]

20ul selling size
Recombinant

ab191416

Anti-FOXP3 antibody [EPR15038-69]

20ul selling size
RabMAb
Recombinant

ab99963

Anti-FOXP3 antibody [SP97]

20ul selling size
RabMAb
Recombinant

ab244242

Anti-FOXP3 antibody [EPR22102-37] - BSA and Azide free

Recombinant
Advanced Validation
RabMAb

ab96048

Anti-FOXP3 antibody [236A/E7] - BSA and Azide free

Advanced Validation
Recombinant

ab289644

Human FOXP3 ELISA Kit

Recombinant
SimpleStep