GCNT1
Function
Glycosyltransferase that catalyzes the transfer of an N-acetylglucosamine (GlcNAc) moiety in beta1-6 linkage from UDP-GlcNAc onto mucin-type core 1 O-glycan to form the branched mucin-type core 2 O-glycan (PubMed:1329093, PubMed:23027862). The catalysis is metal ion-independent and occurs with inversion of the anomeric configuration of sugar donor (By similarity). Selectively involved in synthesis of mucin-type core 2 O-glycans that serve as scaffolds for the display of selectin ligand sialyl Lewis X epitope by myeloid cells, with an impact on homeostasis and recruitment to inflammatory sites (By similarity). Can also act on glycolipid substrates. Transfers GlcNAc moiety to GalGb4Cer globosides in a reaction step to the synthesis of stage-specific embryonic antigen 1 (SSEA-1) determinant (By similarity). Can use Galbeta1-3GalNAcalpha1- and Galbeta1-3GalNAcbeta1- oligosaccharide derivatives as acceptor substrates (By similarity).
Pathway
Protein modification; protein glycosylation.
Glycolipid biosynthesis.
Sequence Similarities
Belongs to the glycosyltransferase 14 family.
Tissue Specificity
Highly expressed in activated T-lymphocytes and myeloid cells.
Cellular localization
- Golgi apparatus membrane
- Single-pass type II membrane protein
- Also detected in the trans-Golgi network.
Alternative names
NACGT2, GCNT1, Core 2-branching enzyme, Core2-GlcNAc-transferase, C2GlcNAcT, C2GNT, Core 2 GNT, C2GnT-L