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GFP

Function

Energy-transfer acceptor. Its role is to transduce the blue chemiluminescence of the protein aequorin into green fluorescent light by energy transfer. Fluoresces in vivo upon receiving energy from the Ca(2+)-activated photoprotein aequorin.

Post-translational modifications

Contains a chromophore consisting of modified amino acid residues. The chromophore is formed by autocatalytic backbone condensation between Ser-65 and Gly-67, and oxidation of Tyr-66 to didehydrotyrosine. Maturation of the chromophore requires nothing other than molecular oxygen.

Sequence similarities

Belongs to the GFP family.

Tissue specificity

Photocytes.

Alternative names

  • Green fluorescent protein
  • GFP

Target type

Proteins

Molecular weight

26886Da