Glutaminase
Domain
A highly mobile activation loop at the dimer-dimer interface is important for enzyme activity.
The C-terminal ANK repeats prevent the assembly of the supra-tetrameric filaments.
Function
Catalyzes the first reaction in the primary pathway for the renal catabolism of glutamine. Plays a role in maintaining acid-base homeostasis. Regulates the levels of the neurotransmitter glutamate, the main excitatory neurotransmitter in the brain.
Post-translational modifications
Synthesized as a 74-kDa cytosolic precursor which is proteolytically processed by the mitochondrial-processing peptidase (MPP) via a 72-kDa intermediate to yield the mature mitochondrial 68- and 65-kDa subunits.
Sequence Similarities
Belongs to the glutaminase family.
Cellular localization
- Isoform 1
- Mitochondrion
- Cytoplasm
- Cytosol
- The 74-kDa cytosolic precursor is translocated into the mitochondria and processed via a 72-kDa intermediate to yield the mature 68- and 65-kDa subunits.
- Isoform 2
- Mitochondrion
- Glutaminase kidney isoform, mitochondrial 68 kDa chain
- Mitochondrion matrix
- Produced by the proteolytic processing of the 74-kDa cytosolic precursor.
- Glutaminase kidney isoform, mitochondrial 65 kDa chain
- Mitochondrion matrix
- Produced by the proteolytic processing of the 74-kDa cytosolic precursor.
Alternative names
Gls1, Kiaa0838, GLS