Glutathione peroxidase 1
GeneName
GPX1
Summary
GPX1, also known as glutathione peroxidase or GPx, is a 22 kDa enzyme that is predominantly expressed in the cytoplasm and mitochondria. It plays a crucial role in protecting cells from oxidative damage by catalysing the reduction of hydrogen peroxide and organic hydroperoxides using glutathione as a substrate. GPX1 is involved in various biological processes, including blood vessel endothelial cell migration, wound healing, and the regulation of redox homeostasis. Additionally, it participates in the intrinsic apoptotic signalling pathway and has implications in cell differentiation and proliferation across multiple tissue types, including muscle and fat cells.
Importance
GPX1 is relevant to: - The study of oxidative stress and its implications in various diseases, including cancer and neurodegenerative disorders, due to its role in detoxifying reactive oxygen species. - Angiogenesis and wound healing, which are critical processes in tissue repair and regeneration. - The regulation of inflammatory responses, as it modulates oxidative stress-induced pathways that can lead to cell apoptosis. - Metabolic processes, particularly those involving lipids and glucose, highlighting its importance in metabolic disorders and cardiovascular health.
Top Products
For researchers investigating GPX1, we recommend two excellent primary antibodies that cater to various experimental needs. The first is the well-cited polyclonal antibody, Anti-Glutathione Peroxidase 1 antibody (ab22604), which has garnered 186 citations, highlighting its reliability in Western blotting (WB) and immunocytochemistry (ICC). This antibody has also been validated in knockout models, ensuring its effectiveness in specific applications.In addition, we offer the recombinant antibody, Anti-Glutathione Peroxidase 1 antibody [EPR3311] (ab108429). This product is validated in knockout models and is suitable for a broader range of applications, including WB, immunohistochemistry (IHC), and flow cytometry (FC). With 16 citations, it demonstrates a growing presence in the research community. The recombinant nature of this antibody ensures batch-to-batch consistency, making it an excellent choice for researchers seeking dependable GPX1 detection. The Glutathione Peroxidase Assay Kit (Colorimetric) (ab102530), with an impressive 142 citations, is an excellent option for researchers looking to accurately measure GPX1 activity in their samples.
Abcam Product Citation Summary
The data indicates that GPX1 is being extensively studied in various contexts related to oxidative stress and inflammation, particularly in Rattus norvegicus and human samples. The use of multiple antibodies for Western blotting highlights the importance of GPX1 in understanding cellular responses to different treatments and conditions, such as dietary influences, mitochondrial metabolism, and injury responses.
Abcam Product Citation Table
Function
Catalyzes the reduction of hydroperoxides in a glutathione-dependent manner thus regulating cellular redox homeostasis (PubMed:11115402, PubMed:36608588). Can reduce small soluble hydroperoxides such as H2O2, cumene hydroperoxide and tert-butyl hydroperoxide, as well as several fatty acid-derived hydroperoxides (PubMed:11115402, PubMed:36608588). In platelets catalyzes the reduction of 12-hydroperoxyeicosatetraenoic acid, the primary product of the arachidonate 12-lipoxygenase pathway (PubMed:11115402).
Post-translational modifications
During periods of oxidative stress, Sec-49 may react with a superoxide radical, irreversibly lose hydroselenide and be converted to dehydroalanine.
Sequence Similarities
Belongs to the glutathione peroxidase family.
Tissue Specificity
Expressed in platelets (at protein level).
Cellular localization
- Cytoplasm
- Mitochondrion
Alternative names
Glutathione peroxidase 1, GPx-1, GSHPx-1, Cellular glutathione peroxidase, Phospholipid-hydroperoxide glutathione peroxidase GPX1, GPX1