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GOPC

GeneName

GOPC

Summary

GOPC, also known as CAL, FIG, or CFTR associated ligand, is a 51 kDa protein that is predominantly expressed in the cytoplasm and is associated with various cellular structures including the Golgi apparatus, dendrites, and postsynaptic densities. It plays a role in intracellular transport processes, particularly in the trafficking of proteins from the Golgi to the plasma membrane, and is involved in the regulation of protein localisation to the cell surface. Additionally, GOPC has been implicated in the negative regulation of anion channel activity and exhibits molecular sequestering activity, indicating its involvement in modulating protein interactions and functions within the cell.

Importance

GOPC is relevant to: - Protein trafficking and localisation, which are essential for maintaining cellular function and communication - Neurobiology due to its expression in dendrites and postsynaptic densities, implicating it in synaptic function and plasticity - Disorders related to protein mislocalisation, such as cystic fibrosis, given its association with CFTR - Golgi apparatus function and its role in vesicular transport, which are critical for secretory pathways in various cell types

Top Products

For researchers investigating GOPC, we recommend two excellent primary antibodies that have garnered attention in the field. The first is the well-cited polyclonal antibody, Anti-PIST antibody (ab37036), which has been referenced in 9 publications and is known for its reliable performance in Western blotting (WB), immunohistochemistry (IHC), and immunocytochemistry (ICC). Additionally, we offer the recombinant antibody, Anti-PIST antibody [EPR4079] (ab109119), which has also been validated for use in WB, IHC, and ICC, and has received 7 citations. This recombinant option provides the added benefit of batch-to-batch consistency, making it an excellent choice for researchers seeking dependable results in their studies of GOPC.

Abcam Product Citation Summary

The use of the Abcam antibody ab37036 for detecting GOPC in mouse models highlights its relevance in studies focused on globozoospermia-related proteins. This suggests a potential role for GOPC in reproductive biology and associated disorders.

Abcam Product Citation Table

Product Code
Species
Application
Study Context
PMID
ab37036
Mouse
WB
globozoospermia-related proteins
28055014

Domain

The PDZ domain mediates interactions with FZD5, FZD8, ASIC3, GRID2, CLCN3 (By similarity). Also mediates interaction with CFTR and ADRB1.

The coiled-coil region probably mediates association to membranes, targeting to the Golgi, and interactions with GOLGA3, and STX6. May also mediate interaction with RHOQ (By similarity).

Function

Plays a role in intracellular protein trafficking and degradation (PubMed:11707463, PubMed:14570915, PubMed:15358775). May regulate CFTR chloride currents and acid-induced ASIC3 currents by modulating cell surface expression of both channels (By similarity). May also regulate the intracellular trafficking of the ADR1B receptor (PubMed:15358775). May play a role in autophagy (By similarity). Together with MARCHF2 mediates the ubiquitination and lysosomal degradation of CFTR (PubMed:23818989). Overexpression results in CFTR intracellular retention and lysosomaldegradation in the lysosomes (PubMed:11707463, PubMed:14570915).

Involvement in disease

A chromosomal aberration involving GOPC is found in a glioblastoma multiforme sample. An intra-chromosomal deletion del(6)(q21q21) is responsible for the formation of GOPC-ROS1 chimeric protein which has a constitutive receptor tyrosine kinase activity.

Tissue Specificity

Ubiquitously expressed.

Cellular localization

Alternative names

CAL, FIG, GOPC, Golgi-associated PDZ and coiled-coil motif-containing protein, CFTR-associated ligand, Fused in glioblastoma, PDZ protein interacting specifically with TC10, PIST

swissprot:Q9HD26 entrezGene:57120 entrezGene:6098 swissprot:P08922 omim:606845 omim:165020