GPT
GeneName
GPT
Summary
GPT, also known as ALT, Gpp, or glutamate pyruvate transaminase, is a 55 kDa enzyme primarily located in the cytosol and cytoplasm, with some presence in extracellular exosomes. It catalyses the transamination reaction between L-alanine and 2-oxoglutarate, producing pyruvate and glutamate, thus playing a vital role in amino acid metabolism and the biosynthesis of various biomolecules. This enzyme is crucial for the catabolism of L-alanine, contributing to energy production and metabolic homeostasis.
Importance
GPT is relevant to: - Metabolic disorders, as it is involved in amino acid metabolism and energy production - Liver function assessments, since elevated levels of GPT are often indicative of liver damage or disease - Research into metabolic pathways, particularly those involving amino acid catabolism and biosynthesis - Understanding the role of transaminases in various physiological and pathological processes
Top Products
For researchers studying GPT, we recommend the well-cited polyclonal antibody, Anti-Alanine Transaminase antibody (ab231715). This antibody is particularly effective for Western blotting (WB) and immunohistochemistry (IHC), making it a reliable choice for various applications in your research. With its citation count of 1, it demonstrates a growing recognition in the scientific community. The ALT Assay Kit (384 well, Colorimetric/Fluorometric) (ab241035) is a reliable option for researchers looking to measure GPT levels, supported by 8 citations.
Abcam Product Citation Summary
The use of the Abcam antibody ab234578 in a humanized liver mouse model highlights its relevance in studying GPT in the context of HBV infection. This suggests a potential role for GPT in liver-related diseases and viral infections.
Abcam Product Citation Table
Function
Catalyzes the reversible transamination between alanine and 2-oxoglutarate to form pyruvate and glutamate. Participates in cellular nitrogen metabolism and also in liver gluconeogenesis starting with precursors transported from skeletal muscles (By similarity).
Pathway
Amino-acid degradation; L-alanine degradation via transaminase pathway; pyruvate from L-alanine: step 1/1.
Sequence Similarities
Belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family. Alanine aminotransferase subfamily.
Tissue Specificity
Liver, kidney, heart, and skeletal muscles. Expressed at moderate levels in the adipose tissue.
Cellular localization
- Cytoplasm
Alternative names
AAT1, GPT1, GPT, Alanine aminotransferase 1, ALT1, Glutamate pyruvate transaminase 1, Glutamic--alanine transaminase 1, Glutamic--pyruvic transaminase 1, GPT 1