HDAC2

GeneName

HDAC2

Summary

HDAC2, also known as histone deacetylase 2 or HD2, is a 55 kDa enzyme that plays a critical role in the regulation of gene expression through histone deacetylation, which leads to chromatin remodelling and transcriptional repression. It is predominantly localised in the nucleus, but can also be found in the cytoplasm, and is involved in various protein complexes, including the NuRD and Sin3-type complexes. HDAC2 interacts with multiple proteins, including transcription factors and heat shock proteins, to modulate cellular processes such as differentiation, apoptosis, and response to various stimuli like dopamine and retinoic acid. Its activity is essential for maintaining cellular homeostasis and regulating developmental processes.

Importance

HDAC2 is relevant to: - Cancer research due to its role in regulating gene expression and cell proliferation, making it a target for histone deacetylase inhibitors in cancer therapy. - Neurodegenerative diseases, as it has been implicated in the regulation of neuronal survival and plasticity. - Cardiovascular diseases through its involvement in cardiac muscle hypertrophy and response to stress. - Stem cell biology, as it plays a role in the regulation of stem cell differentiation and maintenance.

Top Products

For researchers investigating HDAC2, we recommend two excellent primary antibodies. The first is the well-cited polyclonal antibody, Anti-HDAC2 antibody (ab7029), which has garnered 121 citations, reflecting its reliability in immunocytochemistry (ICC) and immunoprecipitation (IP). Additionally, we offer the top-selling recombinant antibody, Anti-HDAC2 antibody [Y461] (ab32117). This monoclonal antibody has been validated in knockout models and is suitable for a wide range of applications, including western blotting (WB), immunohistochemistry (IHC), ICC, immunoprecipitation (IP), and flow cytometry (FC). With 127 citations, it demonstrates strong performance and is an excellent choice for researchers seeking consistency and versatility in their HDAC2 studies.

Abcam Product Citation Summary

The data indicates a significant focus on the role of HDAC2 in various human and mouse cell types, particularly in studies related to gene expression, transcriptional regulation, and the effects of HDAC inhibitors. The use of multiple applications, including Western blotting and ChIP, highlights the importance of HDAC2 in epigenetic regulation and cellular processes such as cilia formation and stress response.

Abcam Product Citation Table

Function

Histone deacetylase that catalyzes the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4) (PubMed:28497810). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events (By similarity). Histone deacetylases act via the formation of large multiprotein complexes (By similarity). Forms transcriptional repressor complexes by associating with MAD, SIN3, YY1 and N-COR (PubMed:12724404). Component of a RCOR/GFI/KDM1A/HDAC complex that suppresses, via histone deacetylase (HDAC) recruitment, a number of genes implicated in multilineage blood cell development (By similarity). Acts as a component of the histone deacetylase NuRD complex which participates in the remodeling of chromatin (PubMed:16428440, PubMed:28977666). Component of the SIN3B complex that represses transcription and counteracts the histone acetyltransferase activity of EP300 through the recognition H3K27ac marks by PHF12 and the activity of the histone deacetylase HDAC2 (PubMed:37137925). Also deacetylates non-histone targets: deacetylates TSHZ3, thereby regulating its transcriptional repressor activity (PubMed:19343227). May be involved in the transcriptional repression of circadian target genes, such as PER1, mediated by CRY1 through histone deacetylation (By similarity). Involved in MTA1-mediated transcriptional corepression of TFF1 and CDKN1A (PubMed:21965678). In addition to protein deacetylase activity, also acts as a protein-lysine deacylase by recognizing other acyl groups: catalyzes removal of (2E)-butenoyl (crotonyl), lactoyl (lactyl) and 2-hydroxyisobutanoyl (2-hydroxyisobutyryl) acyl groups from lysine residues, leading to protein decrotonylation, delactylation and de-2-hydroxyisobutyrylation, respectively (PubMed:28497810, PubMed:29192674, PubMed:35044827).

Post-translational modifications

S-nitrosylated by GAPDH. In neurons, S-nitrosylation at Cys-262 and Cys-274 does not affect enzyme activity, but induces HDAC2 release from chromatin. This in turn increases acetylation of histones surrounding neurotrophin-dependent gene promoters and promotes their transcription. In embryonic cortical neurons, S-Nitrosylation regulates dendritic growth and branching.

Sequence Similarities

Belongs to the histone deacetylase family. HD type 1 subfamily.

Tissue Specificity

Widely expressed; lower levels in brain and lung.

Cellular localization

• Nucleus
• Cytoplasm

Alternative names

Histone deacetylase 2, HD2, Protein deacylase HDAC2, HDAC2

Database links

swissprot:Q92769 omim:605164 omim:601241 entrezGene:3066 entrezGene:3065 swissprot:Q13547