HELQ

Function

Single-stranded 3'-5' DNA helicase that plays a key role in homology-driven double-strand break (DSB) repair (PubMed:11751861, PubMed:19995904, PubMed:21398521, PubMed:24005041, PubMed:24005565, PubMed:34316696, PubMed:34937945). Involved in different DSB repair mechanisms that are guided by annealing of extensive stretches of complementary bases at break ends, such as microhomology-mediated end-joining (MMEJ), single-strand annealing (SSA) or synthesis-dependent strand annealing (SDSA) (PubMed:34937945). Possesses both DNA unwinding and annealing activities (PubMed:34937945). Forms a complex with RAD51, stimulating HELQ DNA helicase activity and ability to unwing DNA (PubMed:34937945). Efficiently unwinds substrates containing 3' overhangs or a D-loop (PubMed:21398521, PubMed:34937945). In contrast, interaction with the replication protein A (RPA/RP-A) complex inhibits DNA unwinding by HELQ but strongly stimulates DNA strand annealing (PubMed:34937945). Triggers displacement of RPA from single-stranded DNA to facilitate annealing of complementary sequences (PubMed:34316696, PubMed:34937945).

Sequence Similarities

Belongs to the helicase family. SKI2 subfamily.

Cellular localization

• Nucleus
• Chromosome
• Localizes to sites of DNA damage; localizes to damaged replication forks.

Alternative names

HEL308, HELQ, Helicase POLQ-like, Mus308-like helicase, POLQ-like helicase

Database links

swissprot:Q8TDG4 swissprot:Q502W9 omim:606769 swissprot:Q05DF9 swissprot:Q96EX7 entrezGene:113510