HIF1AN

Function

Hydroxylates HIF-1 alpha at 'Asn-803' in the C-terminal transactivation domain (CAD). Functions as an oxygen sensor and, under normoxic conditions, the hydroxylation prevents interaction of HIF-1 with transcriptional coactivators including Cbp/p300-interacting transactivator. Involved in transcriptional repression through interaction with HIF1A, VHL and histone deacetylases. Hydroxylates specific Asn residues within ankyrin repeat domains (ARD) of NFKB1, NFKBIA, NOTCH1, ASB4, PPP1R12A and several other ARD-containing proteins. Also hydroxylates Asp and His residues within ARDs of ANK1 and TNKS2, respectively. Negatively regulates NOTCH1 activity, accelerating myogenic differentiation. Positively regulates ASB4 activity, promoting vascular differentiation.

Cellular localization

• Nucleus
• Cytoplasm
• Cytoplasm
• Perinuclear region
• Mainly cytoplasmic localization, but interaction with NOTCH1 results in nuclear localization and interaction with ABPA3 results in perinuclear localization in macrophages.

Alternative names

FIH1, HIF1AN, Hypoxia-inducible factor 1-alpha inhibitor, Factor inhibiting HIF-1, Hypoxia-inducible factor asparagine hydroxylase, FIH-1

Database links

swissprot:Q9NWT6 omim:606615 cloneId:BC007719 entrezGene:55662