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HMOX1

GeneName

HMOX1

Summary

HMOX1, also known as HO-1 or heme oxygenase 1, is a 33 kDa enzyme predominantly expressed in various tissues, particularly in response to oxidative stress. It is located in multiple cellular compartments, including the cytosol, endoplasmic reticulum, and mitochondrial outer membrane. HMOX1 catalyses the degradation of heme into biliverdin, carbon monoxide, and free iron, playing a crucial role in heme catabolism and iron homeostasis. This enzyme is involved in several biological processes, such as angiogenesis, cellular responses to environmental stressors, and the regulation of inflammatory responses, highlighting its importance in maintaining cellular health and function.

Importance

HMOX1 is relevant to: - The regulation of oxidative stress responses, which is vital for cellular protection against damage - Angiogenesis and tissue repair processes, particularly in wound healing and inflammation - The modulation of immune responses through its effects on leukocyte migration and cytokine production - The potential therapeutic targeting in conditions such as cardiovascular diseases, cancer, and neurodegenerative disorders due to its protective roles in cellular stress responses and inflammation

Top Products

For researchers investigating HMOX1, we recommend two excellent primary antibodies. The first is the well-cited Anti-Heme Oxygenase 1 antibody [HO-1-1] (ab13248), a trusted choice for Western blotting (WB), immunohistochemistry (IHC), flow cytometry (FC), and ELISA, with 369 citations highlighting its reliability in the field. Additionally, we offer the recombinant antibody, Anti-Heme Oxygenase 1 antibody [EPR1390Y] (ab68477), which has been validated in knockout models and is suitable for WB, immunocytochemistry (ICC), flow cytometry (FC), and immunoprecipitation (IP). With 272 citations, this recombinant antibody provides the batch-to-batch consistency that many researchers seek. Together, these antibodies represent a robust toolkit for studying HMOX1. The Human Heme Oxygenase 1 ELISA Kit, Fluorescent (ab229429) is an excellent option for researchers looking to measure HMOX1 levels in their samples.

Abcam Product Citation Summary

The data indicates a significant focus on the role of HMOX1 in various biological contexts, particularly in response to oxidative stress, inflammation, and injury across multiple species, including humans and rodents. The use of Abcam antibodies in studies involving liver, kidney, and brain tissues highlights the importance of HMOX1 in cellular protection mechanisms and its potential implications in diseases such as neuroinflammation and oxidative stress-related conditions.

Abcam Product Citation Table

Product Code
Species
Application
Study Context
PMID
ab13243
Rat
IHC-IF
Cerebral ischemia/reperfusion injury
25384416
ab13243
Mouse
WB
Hepatic Nrf2 signaling
29167567
ab13243
Human
WB
Hepatic stress response to acetaminophen
29167567
ab13243
Mouse
WB
Oxidative stress and apoptosis
21752292
ab13243
Rat
IHC-IF
Inflammation
32450903
ab13243
Rat
WB
Intracerebral hemorrhage
32038239
ab13243
Human
WB
Hemolysis
30619356
ab13248
Mouse
WB
Protein expression in response to exercise
23029187
ab13248
Mouse
IHC-IF
Exercise training and antioxidant levels
23029187
ab13248
Mouse
WB
Diabetes mellitus-associated oxidative stress
31244660
ab13248
Mouse
WB
Oxidative stress
25140114
ab13248
Mouse
WB
Activation of antioxidant pathways in radiation-exposed mice
28289414
ab13248
Pig
WB
Peripheral blood monocyte-derived macrophages
28231264
ab13248
Mouse
WB
Regulation of Nrf2 and HO-1
31060323
ab13248
Mouse
WB
Nrf2 pathway's role in retinal protection
31060323
ab13248
Human
WB
Effects of MES and t-BHP
30304831
ab13248
Mouse
WB
Effects of CGA on HO-1 protein induction
28122344
ab13248
Human
IHC
Granulomas
30428359
ab13248
Mouse
IHC
Susceptibility to Mtb infection
30428359
ab137749
Mouse
WB
Effects of oltipraz
31234342
ab137749
Human
WB
NRF2/HMOX1 and NQO-1 pathway activation
30186543
ab189491
Rat
IHC
Mitochondrial damage in neurons
34149393
ab52947
Mouse
WB
Antioxidant enzyme expression
26938529
ab52947
Mouse
IHC
Aging and osteoarthritis
26458773
ab68477
Mouse
WB
Microglial response to LPS stimulation
30271656
ab68477
Rat
WB
Oxidative stress under high glucose conditions
32051471

Domain

The transmembrane domain is necessary for its oligomerization.

Function

Heme oxygenase 1

Catalyzes the oxidative cleavage of heme at the alpha-methene bridge carbon, released as carbon monoxide (CO), to generate biliverdin IXalpha, while releasing the central heme iron chelate as ferrous iron (PubMed:11121422, PubMed:19556236, PubMed:7703255). Affords protection against programmed cell death and this cytoprotective effect relies on its ability to catabolize free heme and prevent it from sensitizing cells to undergo apoptosis (PubMed:20055707).

Heme oxygenase 1

(Microbial infection) During SARS-COV-2 infection, promotes SARS-CoV-2 ORF3A-mediated autophagy but is unlikely to be required for ORF3A-mediated induction of reticulophagy.

Heme oxygenase 1 soluble form

Catalyzes the oxidative cleavage of heme at the alpha-methene bridge carbon, released as carbon monoxide (CO), to generate biliverdin IXalpha, while releasing the central heme iron chelate as ferrous iron.

Involvement in disease

Heme oxygenase 1 deficiency

HMOX1D

A disease characterized by impaired stress hematopoiesis, resulting in marked erythrocyte fragmentation and intravascular hemolysis, coagulation abnormalities, endothelial damage, and iron deposition in renal and hepatic tissues. Clinical features include persistent hemolytic anemia, asplenia, nephritis, generalized erythematous rash, growth retardation and hepatomegaly.

None

The disease is caused by variants affecting the gene represented in this entry.

Post-translational modifications

A soluble form arises by proteolytic removal of the membrane anchor.

Sequence Similarities

Belongs to the heme oxygenase family.

Tissue Specificity

Expressed at higher levels in renal cancer tissue than in normal tissue (at protein level).

Cellular localization

Alternative names

HO, HO1, HMOX1, Heme oxygenase 1, HO-1

swissprot:P09601 entrezGene:3162 omim:141250