HSPB1
GeneName
HSPB1
Summary
HSPB1, also known as p25 or hsp27, is a 23kDa heat shock protein that functions primarily as a chaperone, assisting in protein folding and preventing aggregation. It is expressed in various tissues, including the cytoplasm, nucleus, and cytoskeleton, and is involved in multiple cellular processes such as the cellular response to stress, regulation of apoptosis, and angiogenesis. HSPB1 interacts with a variety of proteins, including kinases and RNA, and plays a role in the transport of proteins along axons. Its diverse functions are crucial for maintaining cellular homeostasis, particularly under stress conditions.
Importance
HSPB1 is relevant to: - Neuroprotection and neurodegenerative diseases due to its role in protein folding and preventing aggregation - Cancer biology, as it is involved in cell survival, angiogenesis, and the regulation of apoptotic pathways - Cardiovascular research, given its influence on endothelial cell migration and response to vascular endothelial growth factor - Inflammatory responses, through its regulation of cytokine production and stress response mechanisms
Top Products
For researchers investigating HSPB1, we highly recommend the top-selling recombinant antibody, Anti-Hsp27 antibody [G3.1] (ab2790). This well-cited antibody has garnered 60 citations, reflecting its strong reputation in the field. It has been validated for use in a variety of applications, including Western blotting (WB), immunohistochemistry (IHC), immunocytochemistry (ICC), and flow cytometry (FC). The recombinant nature of this antibody ensures batch-to-batch consistency, making it an excellent choice for reliable HSPB1 detection in your experiments. The Hsp27 peptide ELISA Kit (ab204861) is an excellent option for researchers looking to measure HSPB1 in their samples.
Abcam Product Citation Summary
The data indicates that HSPB1 is frequently studied in human contexts, particularly in relation to various cancers such as hepatocellular carcinoma and bladder cancer. The use of Abcam antibodies in Western blotting and immunohistochemistry highlights the importance of HSPB1 in understanding disease mechanisms and protein expression in cancer cells and tissues. Additionally, studies involving human monocytes and cell extracts suggest a broader role of HSPB1 in cellular responses and stress.
Abcam Product Citation Table
Function
Small heat shock protein which functions as a molecular chaperone probably maintaining denatured proteins in a folding-competent state (PubMed:10383393, PubMed:20178975). Plays a role in stress resistance and actin organization (PubMed:19166925). Through its molecular chaperone activity may regulate numerous biological processes including the phosphorylation and the axonal transport of neurofilament proteins (PubMed:23728742).
Involvement in disease
Charcot-Marie-Tooth disease, axonal, type 2F
CMT2F
A dominant axonal form of Charcot-Marie-Tooth disease, a disorder of the peripheral nervous system, characterized by progressive weakness and atrophy, initially of the peroneal muscles and later of the distal muscles of the arms. Charcot-Marie-Tooth disease is classified in two main groups on the basis of electrophysiologic properties and histopathology: primary peripheral demyelinating neuropathies (designated CMT1 when they are dominantly inherited) and primary peripheral axonal neuropathies (CMT2). Neuropathies of the CMT2 group are characterized by signs of axonal degeneration in the absence of obvious myelin alterations, normal or slightly reduced nerve conduction velocities, and progressive distal muscle weakness and atrophy. Onset of Charcot-Marie-Tooth disease type 2F is between 15 and 25 years with muscle weakness and atrophy usually beginning in feet and legs (peroneal distribution). Upper limb involvement occurs later.
None
The disease is caused by variants affecting the gene represented in this entry.
Neuronopathy, distal hereditary motor, autosomal dominant 3
HMND3
A form of distal hereditary motor neuronopathy, a heterogeneous group of neuromuscular disorders caused by selective degeneration of motor neurons in the anterior horn of the spinal cord, without sensory deficit in the posterior horn. The overall clinical picture consists of a classical distal muscular atrophy syndrome in the legs without clinical sensory loss. The disease starts with weakness and wasting of distal muscles of the anterior tibial and peroneal compartments of the legs. Later on, weakness and atrophy may expand to the proximal muscles of the lower limbs and/or to the distal upper limbs.
None
The disease is caused by variants affecting the gene represented in this entry.
Post-translational modifications
Phosphorylated upon exposure to protein kinase C activators and heat shock (PubMed:8325890). Phosphorylation by MAPKAPK2 and MAPKAPK3 in response to stress dissociates HSPB1 from large small heat-shock protein (sHsps) oligomers and impairs its chaperone activity and ability to protect against oxidative stress effectively. Phosphorylation by MAPKAPK5 in response to PKA stimulation induces F-actin rearrangement (PubMed:1332886, PubMed:19166925, PubMed:8093612).
Sequence Similarities
Belongs to the small heat shock protein (HSP20) family.
Tissue Specificity
Detected in all tissues tested: skeletal muscle, heart, aorta, large intestine, small intestine, stomach, esophagus, bladder, adrenal gland, thyroid, pancreas, testis, adipose tissue, kidney, liver, spleen, cerebral cortex, blood serum and cerebrospinal fluid. Highest levels are found in the heart and in tissues composed of striated and smooth muscle.
Cellular localization
- Cytoplasm
- Nucleus
- Cytoplasm
- Cytoskeleton
- Spindle
- Cytoplasmic in interphase cells. Colocalizes with mitotic spindles in mitotic cells. Translocates to the nucleus during heat shock and resides in sub-nuclear structures known as SC35 speckles or nuclear splicing speckles.
Alternative names
HSP27, HSP28, HSPB1, Heat shock protein beta-1, HspB1, 28 kDa heat shock protein, Estrogen-regulated 24 kDa protein, Heat shock 27 kDa protein, Heat shock protein family B member 1, Stress-responsive protein 27, HSP 27, SRP27