KATNA1
Domain
The N-terminus is sufficient for interaction with microtubules, although high affinity binding to microtubules also requires an intact C-terminal domain and ATP, which promotes oligomerization.
Function
Catalytic subunit of a complex which severs microtubules in an ATP-dependent manner. Microtubule severing may promote rapid reorganization of cellular microtubule arrays and the release of microtubules from the centrosome following nucleation. Microtubule release from the mitotic spindle poles may allow depolymerization of the microtubule end proximal to the spindle pole, leading to poleward microtubule flux and poleward motion of chromosome. Microtubule release within the cell body of neurons may be required for their transport into neuronal processes by microtubule-dependent motor proteins. This transport is required for axonal growth.
Post-translational modifications
Phosphorylation by DYRK2 triggers ubiquitination and subsequent degradation.
Ubiquitinated by the BCR(KLHL42) E3 ubiquitin ligase complex, leading to its proteasomal degradation. Ubiquitinated by the EDVP E3 ligase complex and subsequently targeted for proteasomal degradation.
Sequence Similarities
Belongs to the AAA ATPase family. Katanin p60 subunit A1 subfamily.
Cellular localization
- Cytoplasm
- Midbody
- Cytoplasm
- Cytoskeleton
- Microtubule organizing center
- Centrosome
- Cytoplasm
- Cytoskeleton
- Spindle pole
- Cytoplasm
- Cytoskeleton
- Spindle
- Predominantly cytoplasmic (PubMed:9658175). Localized diffusely in the cytoplasm during the interphase (PubMed:10751153). During metaphase is localized throughout the cell and more widely dispersed than the microtubules. In anaphase and telophase is localized at the midbody region (PubMed:19261606). Also localized to the interphase centrosome and the mitotic spindle poles (By similarity). Enhanced recruitment to the mitotic spindle poles requires microtubules and interaction with KATNB1 (PubMed:10751153). Localizes within the cytoplasm, partially overlapping with microtubules, in interphase and to the mitotic spindle and spindle poles during mitosis (PubMed:26929214).
Alternative names
Katanin p60 ATPase-containing subunit A1, Katanin p60 subunit A1, p60 katanin, KATNA1