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KCNMA1

Domain

The S0 segment is essential for the modulation by the accessory beta subunits KCNMB1, KCNMB2, KCNMB3 and KCNMB4.

The S4 segment, which is characterized by a series of positively charged amino acids at every third position, is part of the voltage-sensor.

The pore-forming domain (also referred as P region) is imbedded into the membrane, and forms the selectivity filter of the pore. It contains the signature sequence of potassium channels that displays selectivity to potassium.

The RCK N-terminal domain mediates the homotetramerization, thereby promoting the assembly of monomers into functional potassium channel. It includes binding sites for Ca(2+) and Mg(2+) (By similarity).

The calcium bowl constitutes one of the Ca(2+) sensors and probably acts as a Ca(2+)-binding site. There are however other Ca(2+) sensors region required for activation of the channel.

The heme-binding motif mediates inhibition of channel activation by heme. Carbon monoxide-bound heme leads to increased channel activation.

Function

Potassium channel activated by both membrane depolarization or increase in cytosolic Ca(2+) that mediates export of K(+) (PubMed:14523450, PubMed:29330545, PubMed:31152168). It is also activated by the concentration of cytosolic Mg(2+). Its activation dampens the excitatory events that elevate the cytosolic Ca(2+) concentration and/or depolarize the cell membrane. It therefore contributes to repolarization of the membrane potential. Plays a key role in controlling excitability in a number of systems, such as regulation of the contraction of smooth muscle, the tuning of hair cells in the cochlea, regulation of transmitter release, and innate immunity. In smooth muscles, its activation by high level of Ca(2+), caused by ryanodine receptors in the sarcoplasmic reticulum, regulates the membrane potential. In cochlea cells, its number and kinetic properties partly determine the characteristic frequency of each hair cell and thereby helps to establish a tonotopic map. Kinetics of KCNMA1 channels are determined by alternative splicing, phosphorylation status and its combination with modulating beta subunits. Highly sensitive to both iberiotoxin (IbTx) and charybdotoxin (CTX).

Isoform 5

Potassium channel activated by both membrane depolarization or increase in cytosolic Ca(2+) that mediates export of K(+).

Involvement in disease

Paroxysmal nonkinesigenic dyskinesia, 3, with or without generalized epilepsy

PNKD3

An autosomal dominant neurologic disorder characterized by absence seizures, generalized tonic-clonic seizures, paroxysmal nonkinesigenic dyskinesia and involuntary dystonic or choreiform movements. Onset is usually in childhood. Patients may have seizures only, dyskinesia only, or both.

None

The disease is caused by variants affecting the gene represented in this entry.

Epilepsy, idiopathic generalized 16

EIG16

An autosomal dominant form of idiopathic generalized epilepsy, a disorder characterized by recurring generalized seizures in the absence of detectable brain lesions and/or metabolic abnormalities. Generalized seizures arise diffusely and simultaneously from both hemispheres of the brain. Seizure types include juvenile myoclonic seizures, absence seizures, and generalized tonic-clonic seizures. EIG16 is characterized by onset of seizures soon after birth or in the first years of life.

None

Disease susceptibility is associated with variants affecting the gene represented in this entry.

Cerebellar atrophy, developmental delay, and seizures

CADEDS

An autosomal recessive disease characterized by epilepsy, developmental delay and severe cerebellar atrophy.

None

The disease is caused by variants affecting the gene represented in this entry.

Liang-Wang syndrome

LIWAS

An autosomal dominant syndrome characterized by a highly variable phenotype and severity. The broad spectrum of clinical features includes developmental delay, intellectual disability, ataxia, axial hypotonia, and poor or absent speech, visceral and cardiac malformations, connective tissue presentations with arterial involvement, bone dysplasia and characteristic craniofacial dysmorphism. About half of patients have cerebral and cerebellar atrophy, and thin corpus callosum.

None

The disease is caused by variants affecting the gene represented in this entry.

Post-translational modifications

Phosphorylated (Probable). Phosphorylation by kinases such as PKA and/or PKG. In smooth muscles, phosphorylation affects its activity.

Palmitoylation by ZDHHC22 and ZDHHC23 within the intracellular linker between the S0 and S1 transmembrane domains regulates localization to the plasma membrane. Depalmitoylated by LYPLA1 and LYPLAL1, leading to retard exit from the trans-Golgi network.

Sequence Similarities

Belongs to the potassium channel family. Calcium-activated (TC 1.A.1.3) subfamily. KCa1.1/KCNMA1 sub-subfamily.

Tissue Specificity

Widely expressed. Except in myocytes, it is almost ubiquitously expressed.

Cellular localization

Alternative names

KCNMA, SLO, KCNMA1, Calcium-activated potassium channel subunit alpha-1, BK channel, BKCA alpha, K(VCA)alpha, KCa1.1, Maxi K channel, Slo-alpha, Slo1, Slowpoke homolog, MaxiK, Slo homolog, hSlo

swissprot:Q12791 entrezGene:3778 omim:600150