Kdm5b
Developmental stage
Expressed in developing brain, mammary bud, thymus, teeth, whisker follicle, intervertebral disks, olfactory epithelium, eye, stomach and limbs.
Domain
Both the JmjC domain and the JmjN domain are required for enzymatic activity. However ARID and PHD-type 1 domain are not required for activity per se but contributed to recognition of the H3(1-21)K4me2 substrate peptide.
The 2 first PHD-type zinc finger domains are required for transcription repression activity.
Function
Histone demethylase that demethylates 'Lys-4' of histone H3, thereby playing a central role in histone code. Does not demethylate histone H3 'Lys-9' or H3 'Lys-27'. Demethylates trimethylated, dimethylated and monomethylated H3 'Lys-4'. Acts as a transcriptional corepressor for FOXG1B and PAX9. Represses the CLOCK-BMAL1 heterodimer-mediated transcriptional activation of the core clock component PER2.
Sequence Similarities
Belongs to the JARID1 histone demethylase family.
Tissue Specificity
Present at highest levels in testis, where it is enriched in spermatogonia and pachytene cells (at protein level).
Cellular localization
- Nucleus
Alternative names
Jarid1b, Kiaa4034, Plu1, Kdm5b, Lysine-specific demethylase 5B, Histone demethylase JARID1B, Jumonji/ARID domain-containing protein 1B, PLU-1, [histone H3]-trimethyl-L-lysine(4) demethylase 5B