KHSRP
Domain
KH domains KH 3 and KH 4 behave as independent binding modules and can interact with different regions of the AU-rich RNA targets of degradation.
Function
Binds to the dendritic targeting element and may play a role in mRNA trafficking (By similarity). Part of a ternary complex that binds to the downstream control sequence (DCS) of the pre-mRNA. Mediates exon inclusion in transcripts that are subject to tissue-specific alternative splicing. May interact with single-stranded DNA from the far-upstream element (FUSE). May activate gene expression. Also involved in degradation of inherently unstable mRNAs that contain AU-rich elements (AREs) in their 3'-UTR, possibly by recruiting degradation machinery to ARE-containing mRNAs.
Post-translational modifications
Phosphorylation at Ser-193 leads to the unfolding of the unstable KH domain 1, creating a site for 14-3-3 YWHAZ binding, which promotes nuclear localization and impairs the RNA degradation function.
Sequence Similarities
Belongs to the KHSRP family.
Tissue Specificity
Detected in neural and non-neural cell lines.
Cellular localization
- Nucleus
- Cytoplasm
- A small proportion is also found in the cytoplasm of neuronal cell bodies and dendrites.
Alternative names
FUBP2, KHSRP, Far upstream element-binding protein 2, FUSE-binding protein 2, KH type-splicing regulatory protein, p75, KSRP