KLF4
GeneName
KLF4
Summary
KLF4, also known as GKLF, KLF-4, or EZF, is a 55 kDa transcription factor that plays a pivotal role in regulating gene expression across various biological processes. It is predominantly localised in the nucleus and cytoplasm, and is involved in chromatin binding and transcriptional regulation. KLF4 has essential functions in the canonical Wnt signalling pathway, epidermal cell differentiation, and stem cell maintenance. It interacts with various proteins, including beta-catenin and histone deacetylases, to modulate transcriptional activity in response to growth factors and other stimuli. KLF4 is crucial for processes such as cell migration, angiogenesis, and the establishment of the skin barrier, highlighting its significance in developmental and physiological contexts.
Importance
KLF4 is relevant to: - Stem cell biology and reprogramming, as it is one of the factors used to induce pluripotency in somatic cells - Cancer research, due to its role in regulating cell proliferation, differentiation, and apoptosis in various tumour types - Cardiovascular research, as it influences endothelial function and angiogenesis - Developmental biology, given its involvement in morphogenetic processes and tissue differentiation
Top Products
For researchers investigating KLF4, we highly recommend the top-selling recombinant antibody, Anti-KLF4 antibody [EPR19590] (ab215036). This antibody has been validated for a variety of applications, including Western blotting (WB), immunohistochemistry (IHC), immunocytochemistry (ICC), chromatin immunoprecipitation (ChIP), immunoprecipitation (IP), and flow cytometry (FC). With 72 citations, it is well-regarded in the research community, demonstrating its reliability and effectiveness for KLF4 detection. This product is an excellent choice for those seeking consistent performance in their experiments. The Recombinant Human KLF4 protein (denatured) ELISA Kit (ab203545) is an excellent option for researchers looking to study KLF4 in their experiments.
Abcam Product Citation Summary
The data indicates that KLF4 is being studied in various contexts, including endothelial cells, gastric tissues, and mouse models. The use of multiple applications such as Western blotting, immunoprecipitation, and immunohistochemistry highlights the importance of KLF4 in cellular processes like regulation of protein levels, interactions, and responses to environmental factors. The studies span across different species, including humans and mice, suggesting a broad relevance of KLF4 in biological research.
Abcam Product Citation Table
Domain
The 9aaTAD motif is a transactivation domain present in a large number of yeast and animal transcription factors.
Function
Transcription factor; can act both as activator and as repressor. Binds the 5'-CACCC-3' core sequence. Binds to the promoter region of its own gene and can activate its own transcription. Regulates the expression of key transcription factors during embryonic development. Plays an important role in maintaining embryonic stem cells, and in preventing their differentiation. Required for establishing the barrier function of the skin and for postnatal maturation and maintenance of the ocular surface. Involved in the differentiation of epithelial cells and may also function in skeletal and kidney development. Contributes to the down-regulation of p53/TP53 transcription.
Post-translational modifications
Ubiquitinated. 'Lys-48'-linked ubiquitinated and targeted for proteasomal degradation by the SCF(BTRC) E3 ubiquitin-protein ligase complex, thereby negatively regulating cell pluripotency maintenance and embryogenesis.
Polyglutamylated by TTLL1 and TTLL4 at Glu-411, which inhibits KLF4 binding with E3 ligase component BTRC, thereby impeding ubiquitination. Deglutamylated by CCP1 and CCP6; deglutamylation promotes KLF4 ubiquitination. KLF4 glutamylation state plays a critical role in the regulation of its function in cell reprogramming, pluripotency maintenance and embryogenesis.
Sequence Similarities
Belongs to the krueppel C2H2-type zinc-finger protein family.
Cellular localization
- Nucleus
- Cytoplasm
Alternative names
EZF, GKLF, KLF4, Krueppel-like factor 4, Epithelial zinc finger protein EZF, Gut-enriched krueppel-like factor
Database links
swissprot:O43474 entrezGene:9314 omim:602253
Other research areas
- Immunology & Infectious Disease
- Oncology