LAMP1
GeneName
LAMP1
Summary
LAMP1, also known as CD107a, is a 45kDa lysosomal membrane glycoprotein that plays a significant role in the trafficking and function of lysosomes and late endosomes. It is expressed in various immune cells, including natural killer cells and cytotoxic T lymphocytes, where it is involved in the degranulation process, allowing the release of cytotoxic granules. LAMP1 is crucial for the proper localisation of proteins to organelles and is implicated in granzyme-mediated apoptosis and lysosomal lumen acidification. Its presence on the cell surface can also act as a receptor for certain viruses, highlighting its role in immune responses.
Importance
LAMP1 is relevant to: - Immune responses, particularly in natural killer cell activity and cytotoxicity, through its role in degranulation - Lysosomal function and integrity, which are essential for cellular homeostasis and protein degradation - Viral infections, as it may serve as a receptor for some viruses - Autophagy and organelle transport, influencing cellular responses to stress and nutrient availability
Top Products
For researchers investigating LAMP1, we recommend two excellent primary antibodies that cater to various experimental needs. The first is the well-cited polyclonal antibody, Anti-LAMP1 antibody - Lysosome Marker (ab24170), which has garnered 931 citations, highlighting its reliability in Western blotting (WB) and immunohistochemistry (IHC). This antibody is a trusted choice for those looking to study lysosomal markers.In addition, we offer the recombinant antibody, Anti-LAMP1 antibody [EPR4204] - Lysosome Marker (ab108597). This product has been validated in knockout models and is suitable for both WB and IHC applications. With 18 citations, it demonstrates a growing presence in the research community. The recombinant nature of this antibody ensures batch-to-batch consistency, making it an excellent option for researchers seeking dependable results in their studies of LAMP1. The Human LAMP1 ELISA Kit (ab302755) is a reliable option for researchers looking to measure LAMP1 levels in their samples.
Abcam Product Citation Summary
The data indicates a significant focus on the role of LAMP1 in various biological contexts, particularly in studies involving mouse models. Applications include immunocytochemistry, immunofluorescence, and Western blotting, highlighting its importance in understanding lysosomal function, autophagy, and cellular transport mechanisms. The research spans multiple species, including human and rat, and addresses diverse topics such as neurodegenerative diseases, cancer, and cellular stress responses.
Abcam Product Citation Table
Function
Lysosomal membrane glycoprotein which plays an important role in lysosome biogenesis, lysosomal pH regulation, autophagy and cholesterol homeostasis (PubMed:37390818). Acts as an important regulator of lysosomal lumen pH regulation by acting as a direct inhibitor of the proton channel TMEM175, facilitating lysosomal acidification for optimal hydrolase activity (PubMed:37390818). Also plays an important role in NK-cells cytotoxicity (PubMed:2022921, PubMed:23632890). Mechanistically, participates in cytotoxic granule movement to the cell surface and perforin trafficking to the lytic granule (PubMed:23632890). In addition, protects NK-cells from degranulation-associated damage induced by their own cytotoxic granule content (PubMed:23847195). Presents carbohydrate ligands to selectins (PubMed:7685349).
(Microbial infection) Acts as a receptor for Lassa virus glycoprotein (PubMed:24970085, PubMed:25972533, PubMed:27605678, PubMed:28448640). Also promotes fusion of the virus with host membrane in less acidic endosomes (PubMed:29295909).
(Microbial infection) Supports the FURIN-mediated cleavage of mumps virus fusion protein F by interacting with both FURIN and the unprocessed form but not the processed form of the viral protein F.
Post-translational modifications
O- and N-glycosylated; some of the 18 N-linked glycans are polylactosaminoglycans.
(Microbial infection) The glycosylation of Asn-76 is essential for Lassa virus entry into cells.
Sequence Similarities
Belongs to the LAMP family.
Cellular localization
- Lysosome membrane
- Single-pass type I membrane protein
- Endosome membrane
- Single-pass type I membrane protein
- Late endosome membrane
- Single-pass type I membrane protein
- Cell membrane
- Single-pass type I membrane protein
- Cytolytic granule membrane
- Single-pass type I membrane protein
- This protein shuttles between lysosomes, endosomes, and the plasma membrane (By similarity). Colocalizes with OSBPL1A at the late endosome (PubMed:16176980).
Alternative names
CD107a, Lysosome-associated membrane glycoprotein 1, LAMP-1, Lysosome-associated membrane protein 1, CD107 antigen-like family member A, LAMP1
Database links
swissprot:P11279 entrezGene:3916 omim:153330
Other research areas
- Immuno-oncology
- Immunology & Infectious Disease
- Oncology