LAMP2

GeneName

LAMP2

Summary

LAMP2, also known as LAMP-2 or lysosome associated membrane protein 2, is a 45kDa glycoprotein primarily located in the lysosomal membrane and late endosomes. It plays a vital role in autophagy, particularly in chaperone-mediated autophagy, where it facilitates the transport and degradation of proteins within lysosomes. LAMP2 is also involved in the maturation of autophagosomes and contributes to cellular processes such as protein stabilization and targeting to lysosomes. Its expression is observed in various tissues, reflecting its importance in cellular homeostasis and response to stress.

Importance

LAMP2 is relevant to: - Autophagy and lysosomal function, which are critical for cellular health and metabolism - Muscle cell homeostasis, as it is involved in the degradation of damaged proteins - Inflammatory responses through its role in regulating the NLRP3 inflammasome - Diseases related to lysosomal dysfunction, such as Pompe disease and Danon disease, highlighting its significance in therapeutic research

Top Products

For researchers investigating LAMP2, we recommend two excellent primary antibodies. The first is the well-cited Anti-LAMP2 antibody [GL2A7] (ab13524), which has garnered 234 citations and is highly regarded for its performance in Western blotting (WB). This antibody is a reliable choice for those focusing on lysosome markers. Additionally, we offer the recombinant antibody, Anti-LAMP2A antibody [EPR4207(2)] (ab125068), which has been validated in knockout models and is suitable for a broader range of applications, including WB, immunocytochemistry (ICC), immunohistochemistry (IHC), flow cytometry (FC), and immunoprecipitation (IP). With 81 citations, this recombinant product provides the batch-to-batch consistency that many researchers seek, making it an excellent option for comprehensive LAMP2 studies. The Human LAMP2 ELISA Kit (ab302757) is a reliable option for researchers looking to measure LAMP2 levels in their samples.

Abcam Product Citation Summary

The data indicates a significant focus on the role of LAMP2 in various biological contexts, particularly in studies related to mitochondrial function, lysosomal dynamics, and cellular protection mechanisms. The use of LAMP2 antibodies spans multiple species, including human and mouse models, highlighting its relevance in both basic research and potential therapeutic applications. The studies cover a range of applications, primarily Western blotting, indicating a strong interest in protein expression and localization in different tissues and conditions.

Abcam Product Citation Table

Function

Lysosomal membrane glycoprotein which plays an important role in lysosome biogenesis, lysosomal pH regulation and autophagy (PubMed:11082038, PubMed:18644871, PubMed:24880125, PubMed:27628032, PubMed:36586411, PubMed:37390818, PubMed:8662539). Acts as an important regulator of lysosomal lumen pH regulation by acting as a direct inhibitor of the proton channel TMEM175, facilitating lysosomal acidification for optimal hydrolase activity (PubMed:37390818). Plays an important role in chaperone-mediated autophagy, a process that mediates lysosomal degradation of proteins in response to various stresses and as part of the normal turnover of proteins with a long biological half-live (PubMed:11082038, PubMed:18644871, PubMed:24880125, PubMed:27628032, PubMed:36586411, PubMed:8662539). Functions by binding target proteins, such as GAPDH, GPX4, NLRP3 and MLLT11, and targeting them for lysosomal degradation (PubMed:11082038, PubMed:18644871, PubMed:24880125, PubMed:36586411, PubMed:8662539). In the chaperone-mediated autophagy, acts downstream of chaperones, such as HSPA8/HSC70, which recognize and bind substrate proteins and mediate their recruitment to lysosomes, where target proteins bind LAMP2 (PubMed:36586411). Plays a role in lysosomal protein degradation in response to starvation (By similarity). Required for the fusion of autophagosomes with lysosomes during autophagy (PubMed:27628032). Cells that lack LAMP2 express normal levels of VAMP8, but fail to accumulate STX17 on autophagosomes, which is the most likely explanation for the lack of fusion between autophagosomes and lysosomes (PubMed:27628032). Required for normal degradation of the contents of autophagosomes (PubMed:27628032). Required for efficient MHC class II-mediated presentation of exogenous antigens via its function in lysosomal protein degradation; antigenic peptides generated by proteases in the endosomal/lysosomal compartment are captured by nascent MHC II subunits (PubMed:15894275, PubMed:20518820). Is not required for efficient MHC class II-mediated presentation of endogenous antigens (PubMed:20518820).

Isoform LAMP-2C

Modulates chaperone-mediated autophagy. Decreases presentation of endogenous antigens by MHCII. Does not play a role in the presentation of exogenous and membrane-derived antigens by MHCII.

(Microbial infection) Supports the FURIN-mediated cleavage of mumps virus fusion protein F by interacting with both FURIN and the unprocessed form but not the processed form of the viral protein F.

Involvement in disease

Danon disease

DAND

DAND is a lysosomal glycogen storage disease characterized by the clinical triad of cardiomyopathy, vacuolar myopathy and intellectual disability. It is often associated with an accumulation of glycogen in muscle and lysosomes.

None

The disease is caused by variants affecting the gene represented in this entry.

Post-translational modifications

O- and N-glycosylated; some of the 16 N-linked glycans are polylactosaminoglycans.

Sequence Similarities

Belongs to the LAMP family.

Tissue Specificity

Isoform LAMP-2A is highly expressed in placenta, lung and liver, less in kidney and pancreas, low in brain and skeletal muscle (PubMed:26856698, PubMed:7488019). Isoform LAMP-2B is detected in spleen, thymus, prostate, testis, small intestine, colon, skeletal muscle, brain, placenta, lung, kidney, ovary and pancreas and liver (PubMed:26856698, PubMed:7488019). Isoform LAMP-2C is detected in small intestine, colon, heart, brain, skeletal muscle, and at lower levels in kidney and placenta (PubMed:26856698).

Cellular localization

• Lysosome membrane
• Single-pass type I membrane protein
• Endosome membrane
• Single-pass type I membrane protein
• Cell membrane
• Single-pass type I membrane protein
• Cytoplasmic vesicle
• Autophagosome membrane
• This protein shuttles between lysosomes, endosomes, and the plasma membrane.

Alternative names

CD107b, Lysosome-associated membrane glycoprotein 2, LAMP-2, Lysosome-associated membrane protein 2, CD107 antigen-like family member B, LGP-96, LAMP2

Database links

swissprot:P13473 entrezGene:3920 omim:309060

Other research areas

• Immuno-oncology
• Oncology