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LDHA

GeneName

LDHA

Summary

LDHA, also known as D lactate dehydrogenase or LDH A, is a 37 kDa enzyme that catalyses the conversion of pyruvate to lactate in the glycolytic pathway, facilitating anaerobic metabolism. It is primarily expressed in tissues with high glycolytic activity, such as muscle and brain, and is localised in the cytosol and mitochondria, among other cellular compartments. LDHA plays a crucial role in the lactate metabolic process and is involved in various biological processes including glucose catabolism and the development of the substantia nigra. Its activity is essential for maintaining energy production under hypoxic conditions and is linked to the regulation of cellular metabolism.

Importance

LDHA is relevant to: - Cancer metabolism, as it supports the Warburg effect where cancer cells preferentially convert glucose to lactate even in the presence of oxygen - Metabolic disorders, including lactic acidosis, due to its role in lactate production and clearance - Neurodegenerative diseases, particularly in the context of neuronal energy metabolism and the development of the substantia nigra - Exercise physiology, since it is involved in muscle fatigue and recovery processes during intense physical activity

Top Products

For researchers investigating LDHA, we highly recommend the top-selling recombinant antibody, Anti-Lactate Dehydrogenase antibody [EPR1564] (ab101562). This well-cited antibody has garnered 86 citations, reflecting its reliability and trust within the scientific community. It has been validated for use in Western blotting (WB) and immunohistochemistry (IHC), making it a versatile choice for various experimental needs. The recombinant nature of this antibody ensures batch-to-batch consistency, providing researchers with confidence in their results. The Anti-Lactate Dehydrogenase antibody [98A-1F9BB1] ELISA Kit (ab135396), supported by 5 citations, is an excellent option for researchers looking to accurately measure LDHA levels in their samples.

Abcam Product Citation Summary

The data indicates that LDHA is being studied in various contexts, particularly in relation to heart failure, glycometabolic processes, and obesity. The use of different species, including rats, mice, and humans, highlights the relevance of LDHA in metabolic and cancer research.

Abcam Product Citation Table

Product Code
Species
Application
Study Context
PMID
ab101562
Rat
WB
Heart failure
32158496
ab101562
Mouse
WB
Glycometabolic-related proteins
32076440
ab134187
Mouse
WB
Liver samples
27013658
ab47010
Human
WB
Correlation between LDHB expression and taxol potency
25973606
ab47010
Human
WB
Efficacy of taxol
25973606
ab47010
Mouse
IF
Obesity
32221369
ab47010
Mouse
IF
Obesity
32221369

Function

Interconverts simultaneously and stereospecifically pyruvate and lactate with concomitant interconversion of NADH and NAD(+).

Involvement in disease

Glycogen storage disease 11

GSD11

A metabolic disorder that results in exertional myoglobinuria, pain, cramps and easy fatigue.

None

The disease is caused by variants affecting the gene represented in this entry.

Pathway

Fermentation; pyruvate fermentation to lactate; (S)-lactate from pyruvate: step 1/1.

Post-translational modifications

ISGylated.

Sequence Similarities

Belongs to the LDH/MDH superfamily. LDH family.

Tissue Specificity

Predominantly expressed in anaerobic tissues such as skeletal muscle and liver.

Cellular localization

Alternative names

PIG19, LDHA, L-lactate dehydrogenase A chain, LDH-A, Cell proliferation-inducing gene 19 protein, LDH muscle subunit, Renal carcinoma antigen NY-REN-59, LDH-M

swissprot:P00338 omim:150000 omim:150100 swissprot:P07195 swissprot:P07864 entrezGene:3939 entrezGene:3945

Other research areas