LDHB

GeneName

LDHB

Summary

LDHB, also known as lactate dehydrogenase B or LDH B, is a 37 kDa enzyme that catalyses the conversion of lactate to pyruvate in the cytoplasm and mitochondria, playing a vital role in the lactate and pyruvate metabolic processes. It is primarily expressed in tissues with high glycolytic activity, such as skeletal muscle and the heart. LDHB is involved in the regulation of cellular energy metabolism and is part of the oxidoreductase complex, with activity dependent on NAD binding. The enzyme can also be found in extracellular exosomes and membrane rafts, indicating its potential roles in intercellular communication and metabolic regulation.

Importance

LDHB is relevant to: - Cancer metabolism, as it influences the Warburg effect and lactate production in tumours - Exercise physiology, due to its role in muscle metabolism and recovery - Metabolic disorders, where alterations in lactate metabolism can indicate pathological states - Cardiovascular health, given its involvement in heart muscle energy metabolism and response to ischaemia

Top Products

For researchers investigating LDHB, we highly recommend the top-selling recombinant antibody, Anti-Lactate Dehydrogenase antibody [EP1566Y] (ab52488). This antibody has been validated for a variety of applications, including Western blotting (WB), immunohistochemistry (IHC), immunocytochemistry (ICC), flow cytometry (FC), and immunoprecipitation (IP). With 140 citations, it is well-regarded in the research community for its reliability and performance. This makes it an excellent choice for those seeking consistent and effective detection of LDHB in their studies. The Anti-Lactate Dehydrogenase B/LDH-B antibody ELISA Kit (ab53292), supported by 24 citations, is an excellent option for researchers looking to accurately measure LDHB levels in their samples.

Abcam Product Citation Summary

The data indicates that the LDHB antibody (ab52488) has been extensively used in various studies involving different species, primarily focusing on human and mouse tissues. The applications predominantly include Western blotting, highlighting its role in research related to apoptosis, liver injuries, and muscle changes. The studies encompass a range of biological contexts, particularly in heart and skeletal muscle tissues, as well as human cell lines.

Abcam Product Citation Table

Function

Interconverts simultaneously and stereospecifically pyruvate and lactate with concomitant interconversion of NADH and NAD(+).

Involvement in disease

Lactate dehydrogenase B deficiency

LDHBD

A condition with no deleterious effects on health. LDHBD is of interest to laboratory medicine mainly because it can cause misdiagnosis in those disorders in which elevation of serum LDH is expected.

None

The disease is caused by variants affecting the gene represented in this entry.

Pathway

Fermentation; pyruvate fermentation to lactate; (S)-lactate from pyruvate: step 1/1.

Sequence Similarities

Belongs to the LDH/MDH superfamily. LDH family.

Tissue Specificity

Predominantly expressed in aerobic tissues such as cardiac muscle.

Cellular localization

• Cytoplasm
• Mitochondrion inner membrane
• Peripheral membrane protein

Alternative names

L-lactate dehydrogenase B chain, LDH-B, LDH heart subunit, Renal carcinoma antigen NY-REN-46, LDH-H, LDHB

Database links

swissprot:P07195 omim:150000 omim:150100 swissprot:P00338 entrezGene:3939 swissprot:P07864 entrezGene:3945

Other research areas

• Immunology & Infectious Disease
• Oncology