LIPE
GeneName
LIPE
Summary
LIPE, also known as hormone sensitive lipase (HSL), is a 117 kDa enzyme primarily expressed in adipose tissue and other lipid-rich tissues. It is localised to various cellular compartments including the cytosol, lipid droplets, and membrane structures such as caveolae. LIPE plays a crucial role in lipid metabolism by hydrolysing stored triglycerides into free fatty acids and glycerol, and is involved in the catabolism of diacylglycerols and monoacylglycerols. Its enzymatic activities include diacylglycerol lipase, monoacylglycerol lipase, and triacylglycerol lipase, contributing to the regulation of energy homeostasis and lipid signalling pathways.
Importance
LIPE is relevant to: - Metabolic disorders such as obesity and diabetes due to its role in lipolysis and energy balance - Cardiovascular health, as it influences lipid profiles and atherosclerosis risk - Hormonal regulation of lipid metabolism, particularly in response to insulin and catecholamines - The study of lipid-related diseases, including fatty liver disease and dyslipidaemias, highlighting its therapeutic potential
Top Products
For researchers investigating LIPE, we recommend two excellent primary antibodies that cater to various experimental needs. The first is the well-cited polyclonal antibody, Anti-Hormone sensitive lipase/HSL antibody (ab45422), which has garnered 55 citations, highlighting its reliability in Western blotting (WB) and immunocytochemistry (ICC). This antibody is a trusted choice for those looking to study hormone-sensitive lipase in detail. Additionally, we offer the recombinant monoclonal antibody, Anti-Hormone sensitive lipase/HSL antibody [EPR27268-74] (ab322344). This product is validated for use in Western blotting (WB) and immunohistochemistry (IHC), providing researchers with the batch-to-batch consistency that recombinant antibodies are known for. Together, these antibodies provide a robust toolkit for studying LIPE effectively.
Abcam Product Citation Summary
The data indicates that the LIPE gene, which is involved in lipolysis, has been studied using Abcam antibody ab45422 in rat models. This suggests a focus on understanding the mechanisms of fat metabolism and its implications in various physiological and pathological conditions.
Abcam Product Citation Table
Function
Lipase with broad substrate specificity, catalyzing the hydrolysis of triacylglycerols (TAGs), diacylglycerols (DAGs), monoacylglycerols (MAGs), cholesteryl esters and retinyl esters (PubMed:15716583, PubMed:15955102, PubMed:19800417, PubMed:8812477). Shows a preferential hydrolysis of DAGs over TAGs and MAGs and preferentially hydrolyzes the fatty acid (FA) esters at the sn-3 position of the glycerol backbone in DAGs (PubMed:19800417). Preferentially hydrolyzes FA esters at the sn-1 and sn-2 positions of the glycerol backbone in TAGs (By similarity). Catalyzes the hydrolysis of 2-arachidonoylglycerol, an endocannabinoid and of 2-acetyl monoalkylglycerol ether, the penultimate precursor of the pathway for de novo synthesis of platelet-activating factor (By similarity). In adipose tissue and heart, it primarily hydrolyzes stored triglycerides to free fatty acids, while in steroidogenic tissues, it principally converts cholesteryl esters to free cholesterol for steroid hormone production (By similarity).
Involvement in disease
Lipodystrophy, familial partial, 6
FPLD6
An autosomal recessive form of lipodystrophy characterized by abnormal subcutaneous fat distribution. Affected individuals have increased visceral fat, impaired lipolysis, dyslipidemia, hepatic steatosis, systemic insulin resistance, and diabetes. Some patients manifest muscular dystrophy.
None
The disease is caused by variants affecting the gene represented in this entry.
Pathway
Glycerolipid metabolism; triacylglycerol degradation.
Post-translational modifications
Phosphorylation by AMPK reduces its translocation towards the lipid droplets.
Sequence Similarities
Belongs to the 'GDXG' lipolytic enzyme family.
Tissue Specificity
Testis.
Cellular localization
- Cell membrane
- Membrane
- Caveola
- Cytoplasm
- Cytosol
- Lipid droplet
- Found in the high-density caveolae. Translocates to the cytoplasm from the caveolae upon insulin stimulation (PubMed:17026959). Phosphorylation by AMPK reduces its translocation towards the lipid droplets (By similarity).
Alternative names
Hormone-sensitive lipase, HSL, Monoacylglycerol lipase LIPE, Retinyl ester hydrolase, REH, LIPE
Database links
swissprot:Q05469 swissprot:Q6NSL7 entrezGene:3991 omim:151750