LNX1
Domain
The NPXY motif is required for the interaction with the PID domain of NUMB. It is however not sufficient.
The PDZ 1 domain participates in the interaction with the PID domain of NUMB, and participates in the isoform-specific ubiquitination of NUMB. The PDZ 2 domain of isoform 2 participates in the interaction with IGSF5/JAM4, other isoforms containing this domain may also interact with IGSF5/JAM4 (By similarity).
Function
E3 ubiquitin-protein ligase that mediates ubiquitination and subsequent proteasomal degradation of NUMB. E3 ubiquitin ligases accept ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Mediates ubiquitination of isoform p66 and isoform p72 of NUMB, but not that of isoform p71 or isoform p65.
Isoform 2 provides an endocytic scaffold for IGSF5/JAM4.
Pathway
Protein modification; protein ubiquitination.
Tissue Specificity
Expressed in heart, placenta, kidney, pancreas and brain.
Cellular localization
- Cytoplasm
Alternative names
LNX, PDZRN2, UNQ574/PRO1136, LNX1, E3 ubiquitin-protein ligase LNX, Ligand of Numb-protein X 1, Numb-binding protein 1, PDZ domain-containing RING finger protein 2, RING-type E3 ubiquitin transferase LNX