MCL1
GeneName
MCL1
Summary
MCL1, also known as MCL-1, is a 37kDa anti-apoptotic protein that belongs to the Bcl-2 family. It is primarily localised to the mitochondrial outer membrane but is also found in the cytoplasm, nucleoplasm, and nucleus. MCL1 plays a crucial role in regulating cell survival by inhibiting apoptosis through various mechanisms, including binding to pro-apoptotic proteins and modulating mitochondrial dynamics. It is involved in several biological processes such as cellular homeostasis, DNA damage response, and the regulation of apoptotic pathways, making it essential for cell fate determination and response to stress signals.
Importance
MCL1 is relevant to: - Cancer biology, as its overexpression is often associated with tumour survival and resistance to therapy - Neurodegenerative diseases, given its role in regulating neuronal apoptosis and response to oxidative stress - Cellular responses to cytokines, impacting immune cell function and inflammation - Mitochondrial dynamics and function, influencing processes like mitochondrial fusion and cytochrome c release
Top Products
For researchers investigating MCL1, we highly recommend the top-selling recombinant antibody, Anti-MCL1 antibody [Y37] (ab32087). This well-cited product has garnered 176 citations, reflecting its strong reputation in the field. It has been validated in knockout models and is suitable for a variety of applications, including Western blotting (WB), immunohistochemistry (IHC), immunocytochemistry (ICC), and flow cytometry (FC). This versatility makes it an excellent choice for those seeking reliable and consistent results in their MCL1 studies. The Anti-MCL1 antibody [Y37] ELISA Kit (ab32087), with 176 citations, is an excellent option for researchers looking to accurately measure MCL1 levels in their samples.
Abcam Product Citation Summary
The data indicates that the MCL1 target is extensively studied in various human cancer cell lines, particularly in the context of mitochondrial biogenesis, cell migration, and sensitivity to treatments like taxanes. The use of the Abcam antibody ab32087 in both Western blotting and immunohistochemistry highlights its versatility in different experimental setups, including studies on hyperosmotic conditions and TRAIL signaling pathways.
Abcam Product Citation Table
Function
Involved in the regulation of apoptosis versus cell survival, and in the maintenance of viability but not of proliferation. Mediates its effects by interactions with a number of other regulators of apoptosis. Isoform 1 inhibits apoptosis. Isoform 2 promotes apoptosis.
Post-translational modifications
Cleaved by CASP3 during apoptosis. In intact cells cleavage occurs preferentially after Asp-127, yielding a pro-apoptotic 28 kDa C-terminal fragment.
Rapidly degraded in the absence of phosphorylation on Thr-163 in the PEST region.
Phosphorylated on Ser-159, by GSK3, in response to IL3/interleukin-3 withdrawal. Phosphorylation at Ser-159 induces ubiquitination and proteasomal degradation, abrogating the anti-apoptotic activity. Treatment with taxol or okadaic acid induces phosphorylation on additional sites.
Ubiquitinated. Ubiquitination is induced by phosphorylation at Ser-159 (PubMed:16543145). Deubiquitinated by USP20; leading to increased stability (PubMed:35063767).
Sequence Similarities
Belongs to the Bcl-2 family.
Cellular localization
- Membrane
- Single-pass membrane protein
- Cytoplasm
- Mitochondrion
- Nucleus
- Nucleoplasm
- Cytoplasmic, associated with mitochondria.
Alternative names
BCL2L3, MCL1, Induced myeloid leukemia cell differentiation protein Mcl-1, Bcl-2-like protein 3, Bcl-2-related protein EAT/mcl1, mcl1/EAT, Bcl2-L-3