MMP1
GeneName
MMP1
Summary
MMP1, also known as collagenase or matrix metalloproteinase-1, is a 54 kDa enzyme that is secreted into the extracellular matrix and extracellular space. It plays a pivotal role in the breakdown of collagen and other components of the extracellular matrix, facilitating processes such as extracellular matrix disassembly and organisation. MMP1 exhibits endopeptidase and metalloendopeptidase activities, with a specific ability to bind zinc ions, which is essential for its catalytic function. This protein is involved in various biological processes, including the cellular response to UV-A and proteolysis.
Importance
MMP1 is relevant to: - Tissue remodelling and repair, as it regulates the turnover of extracellular matrix components - Cancer progression, due to its role in facilitating tumour invasion and metastasis through matrix degradation - Inflammatory diseases, where it contributes to the pathological remodelling of tissues - Ageing processes, as its activity is associated with the degradation of collagen in skin and other tissues.
Top Products
For researchers investigating MMP1, we recommend two excellent primary antibodies that cater to different experimental needs. The first is the well-cited polyclonal antibody, Anti-MMP1 antibody (ab137332), which has garnered 89 citations and is particularly effective for Western blotting (WB). This product is widely trusted in the research community for its reliability in detecting MMP1.In addition, we offer the recombinant antibody, Anti-MMP1 antibody [EP1247Y] (ab52631), which has also received substantial recognition with 83 citations. This recombinant antibody is validated for multiple applications, including immunohistochemistry (IHC), immunocytochemistry (ICC), and flow cytometry (FC). Its recombinant nature ensures batch-to-batch consistency, making it an excellent choice for researchers seeking dependable results across various experimental setups. The Recombinant Human MMP1 protein ELISA Kit (ab283424) is an excellent option for researchers looking to measure MMP1 levels in their samples.
Abcam Product Citation Summary
The data indicates that MMP1 is being studied in various contexts, particularly in relation to aging and tissue remodeling. Mouse models are frequently used, especially in skin and fibrosis studies, while human tissues are also examined, particularly in the context of tendon ruptures and treatment effects. The applications of Western blotting and immunohistochemistry are prominent in these studies, highlighting the importance of MMP1 in understanding biological processes and potential therapeutic interventions.
Abcam Product Citation Table
Domain
There are two distinct domains in this protein; the catalytic N-terminal, and the C-terminal which is involved in substrate specificity and in binding TIMP (tissue inhibitor of metalloproteinases).
The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
Function
Cleaves collagens of types I, II, and III at one site in the helical domain. Also cleaves collagens of types VII and X (PubMed:1645757, PubMed:2153297, PubMed:2557822). In case of HIV infection, interacts and cleaves the secreted viral Tat protein, leading to a decrease in neuronal Tat's mediated neurotoxicity (PubMed:16807369).
Post-translational modifications
Undergoes autolytic cleavage to two major forms (22 kDa and 27 kDa). A minor form (25 kDa) is the glycosylated form of the 22 kDa form. The 27 kDa form has no activity while the 22/25 kDa form can act as activator for collagenase.
Tyrosine phosphorylated in platelets by PKDCC/VLK.
Sequence Similarities
Belongs to the peptidase M10A family.
Cellular localization
- Secreted
- Extracellular space
- Extracellular matrix
Alternative names
CLG, MMP1, Interstitial collagenase, Fibroblast collagenase, Matrix metalloproteinase-1, MMP-1