MS4A1
GeneName
MS4A1
Summary
MS4A1, also known as CD20 or Bp35, is a 33kDa transmembrane protein predominantly expressed on the surface of B cells. It is localised to the plasma membrane and plays a crucial role in B cell activation, differentiation, and proliferation. MS4A1 is involved in the B cell receptor signalling pathway and interacts with various proteins, including MHC class II molecules and immunoglobulins. This protein is also implicated in calcium ion import into the cytosol, which is essential for various cellular processes, including the humoral immune response.
Importance
MS4A1 is relevant to: - B cell malignancies, such as non-Hodgkin lymphoma, where it serves as a therapeutic target for monoclonal antibodies like rituximab - Understanding B cell biology and the mechanisms of immune response, particularly in autoimmune diseases - Investigating calcium signalling pathways in immune cells, which are critical for cell activation and function - The development of vaccines and immunotherapies aimed at enhancing B cell responses
Top Products
For researchers investigating MS4A1, we recommend two excellent primary antibodies. The first is the well-cited Anti-CD20 antibody [L26] (ab9475), which has garnered 99 citations and is particularly effective for immunohistochemistry (IHC). This antibody is a trusted choice for those focusing on MS4A1 research. Additionally, we offer the recombinant antibody, Anti-CD20 antibody [EP459Y] (ab78237). This product stands out with 116 citations and has been validated in knockout models, making it suitable for a variety of applications including flow cytometry (FC), IHC, immunocytochemistry (ICC), western blotting (WB), and immunoprecipitation (IP). The recombinant nature of this antibody ensures batch-to-batch consistency, providing researchers with reliable results in their studies of MS4A1. The PE/DyLight™ 594 Anti-CD20 antibody [2H7] ELISA Kit (ab187593) is an excellent option for researchers looking to study MS4A1.
Abcam Product Citation Summary
The data indicates that MS4A1 is being studied in the context of various human cancers, particularly in oral squamous cell carcinoma (OSCC) and nasopharyngeal carcinoma. The use of immunohistochemistry suggests a focus on the protein's expression in these tissues, which may have implications for understanding the role of MS4A1 in cancer pathology.
Abcam Product Citation Table
Function
B-lymphocyte-specific membrane protein that plays a role in the regulation of cellular calcium influx necessary for the development, differentiation, and activation of B-lymphocytes (PubMed:12920111, PubMed:3925015, PubMed:7684739). Functions as a store-operated calcium (SOC) channel component promoting calcium influx after activation by the B-cell receptor/BCR (PubMed:12920111, PubMed:18474602, PubMed:7684739).
Involvement in disease
Immunodeficiency, common variable, 5
CVID5
A primary immunodeficiency characterized by antibody deficiency, hypogammaglobulinemia, recurrent bacterial infections and an inability to mount an antibody response to antigen. The defect results from a failure of B-cell differentiation and impaired secretion of immunoglobulins; the numbers of circulating B-cells is usually in the normal range, but can be low.
None
The disease is caused by variants affecting the gene represented in this entry.
Post-translational modifications
Phosphorylated on serines and threonines in resting B-cells. Protein kinase C/PKC can use CD20 as substrate.
Sequence Similarities
Belongs to the MS4A family.
Tissue Specificity
Expressed on B-cells.
Cellular localization
- Cell membrane
- Multi-pass membrane protein
- Cell membrane
- Lipid-anchor
- Constitutively associated with membrane rafts.
Alternative names
CD20, MS4A1, B-lymphocyte antigen CD20, B-lymphocyte surface antigen B1, Bp35, Leukocyte surface antigen Leu-16, Membrane-spanning 4-domains subfamily A member 1
Database links
swissprot:P11836 omim:112210 entrezGene:931
Other research areas
- Immuno-oncology
- Neuroscience