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MT-CO1

GeneName

MT-CO1

Summary

MT-CO1, also known as COI or cytochrome c oxidase subunit 1, is a 57 kDa protein that is an essential component of the mitochondrial inner membrane, specifically part of the respiratory chain complex IV. It plays a crucial role in aerobic respiration and cellular respiration by facilitating the transfer of electrons from cytochrome c to oxygen, thereby contributing to the mitochondrial electron transport chain. MT-CO1 is involved in various biological processes, including cerebellum development and responses to copper ions, electrical stimuli, hypoxia, and oxidative stress.

Importance

MT-CO1 is relevant to: - Mitochondrial function and energy production, as it is integral to the respiratory electron transport chain - Disorders related to mitochondrial dysfunction, which can lead to a range of diseases including neurodegenerative disorders and metabolic syndromes - Research on hypoxia and oxidative stress responses, providing insights into cellular adaptation mechanisms - Studies on copper ion homeostasis, given its response to copper levels in the cellular environment

Top Products

For researchers investigating MT-CO1, we highly recommend the top-selling recombinant antibody, Anti-MTCO1 antibody [1D6E1A8] (ab14705). This well-cited product has garnered 591 citations, reflecting its reliability and trust within the scientific community. It has been validated for use in several applications, including Western blotting (WB), immunohistochemistry (IHC), immunocytochemistry (ICC), and flow cytometry (FC). The recombinant nature of this antibody ensures batch-to-batch consistency, making it an excellent choice for your research needs. The MitoBiogenesis™ In-Cell ELISA Kit (Fluorescent) for MT-CO1 (ab140359), supported by 2 citations, offers a reliable method for researchers looking to assess mitochondrial biogenesis in their samples.

Abcam Product Citation Summary

The data indicates that the Abcam antibody ab14705 is widely used for detecting MT-CO1 in various species, particularly in mouse and human models. The studies focus on diverse contexts such as mitochondrial function, neurodegeneration, and the effects of chemotherapy on mitochondrial dynamics. The applications primarily involve Western blotting and immunohistochemistry, highlighting the importance of MT-CO1 in understanding mitochondrial-related diseases and conditions.

Abcam Product Citation Table

ab14705
Human
WB
Fibroblasts
25852744
ab14705
Mouse
WB
SH-SY5Y neuroblastoma cells
26971449
ab14705
Mouse
WB
Neuronal mitochondrial function
26971449
ab14705
Mouse
WB
Neurodegeneration
26971449
ab14705
Mouse
WB, IHC
Stabilization of PGC-1α by necdin
26971449
ab14705
Mouse
WB, IHC
Cortical neurons
26971449
ab14705
Mouse
WB
Mitochondrial biomass
28871145
ab14705
Mouse
WB
Thermogenic and oxidative gene expression
28515438
ab14705
Mouse
WB
Respiratory capacity
28515438
ab14705
Human
WB
Myoblasts
30262910
ab14705
Rat
WB
Ischemia-reperfusion injury
31443530
ab14705
Human
WB
COX7RP-MCF7 cells under hypoxic conditions
31511525
ab14705
Mouse
WB
Effects of training and Parkin
29549884
ab14705
Mouse
WB
Mitochondrial adaptations following voluntary wheel training
29549884
ab14705
Mouse
WB
Metformin treatment
23166782
ab14705
Mouse
WB, IHC
Chemotherapy treatment-induced mitochondrial functional changes in skeletal muscle
25732599
ab14705
Mouse
WB, IHC
Chemotherapy treatment-induced mitochondrial functional changes
25732599
ab14705
Human
WB
Cell lines
32161263
ab14705
Rat
WB
Retinal cell lysates
31337818
ab14705
Mouse
WB
Mitochondrial function
28821867
ab14705
Human
WB
PC3 cells
27248169
ab14705
Human
WB
Muscle samples
32158465
ab14705
Human
IF
Muscle fibers
30014514
ab14705
Human
IHC-IF, ICC-IF
Muscle tissue
30014514
ab14705
Mouse
WB
Dopaminergic neuron degeneration
26971449
ab14705
Mouse
WB
Parkinson's disease
26971449
ab14705
Mouse
WB
Mitochondrial content
33170774
ab14705
Human
IF
RPE1 cells
26110381

Function

Component of the cytochrome c oxidase, the last enzyme in the mitochondrial electron transport chain which drives oxidative phosphorylation. The respiratory chain contains 3 multisubunit complexes succinate dehydrogenase (complex II, CII), ubiquinol-cytochrome c oxidoreductase (cytochrome b-c1 complex, complex III, CIII) and cytochrome c oxidase (complex IV, CIV), that cooperate to transfer electrons derived from NADH and succinate to molecular oxygen, creating an electrochemical gradient over the inner membrane that drives transmembrane transport and the ATP synthase. Cytochrome c oxidase is the component of the respiratory chain that catalyzes the reduction of oxygen to water. Electrons originating from reduced cytochrome c in the intermembrane space (IMS) are transferred via the dinuclear copper A center (CU(A)) of subunit 2 and heme A of subunit 1 to the active site in subunit 1, a binuclear center (BNC) formed by heme A3 and copper B (CU(B)). The BNC reduces molecular oxygen to 2 water molecules using 4 electrons from cytochrome c in the IMS and 4 protons from the mitochondrial matrix.

Involvement in disease

Leber hereditary optic neuropathy

LHON

A maternally inherited form of Leber hereditary optic neuropathy, a mitochondrial disease resulting in bilateral painless loss of central vision due to selective degeneration of the retinal ganglion cells and their axons. The disorder shows incomplete penetrance and male predominance. Cardiac conduction defects and neurological defects have also been described in some LHON patients. LHON results from primary mitochondrial DNA mutations affecting the respiratory chain complexes.

None

The disease is caused by variants affecting the gene represented in this entry.

MT-CO1 may play a role in the pathogenesis of acquired idiopathic sideroblastic anemia, a disease characterized by inadequate formation of heme and excessive accumulation of iron in mitochondria. Mitochondrial iron overload may be attributable to mutations of mitochondrial DNA because these can cause respiratory chain dysfunction, thereby impairing reduction of ferric iron to ferrous iron. The reduced form of iron is essential to the last step of mitochondrial heme biosynthesis.

Mitochondrial complex IV deficiency

MT-C4D

A disorder of the mitochondrial respiratory chain with heterogeneous clinical manifestations, ranging from isolated myopathy to severe multisystem disease affecting several tissues and organs. Features include hypertrophic cardiomyopathy, hepatomegaly and liver dysfunction, hypotonia, muscle weakness, exercise intolerance, developmental delay, delayed motor development and intellectual disability. Some affected individuals manifest a fatal hypertrophic cardiomyopathy resulting in neonatal death. A subset of patients manifest Leigh syndrome.

None

The disease is caused by variants affecting the gene represented in this entry.

Recurrent myoglobinuria mitochondrial

RM-MT

Recurrent myoglobinuria is characterized by recurrent attacks of rhabdomyolysis (necrosis or disintegration of skeletal muscle) associated with muscle pain and weakness, and followed by excretion of myoglobin in the urine.

None

The gene represented in this entry may be involved in disease pathogenesis.

Deafness, sensorineural, mitochondrial

DFNM

A form of non-syndromic deafness with maternal inheritance. Affected individuals manifest progressive, postlingual, sensorineural hearing loss involving high frequencies.

None

The disease is caused by variants affecting the gene represented in this entry.

Colorectal cancer

CRC

A complex disease characterized by malignant lesions arising from the inner wall of the large intestine (the colon) and the rectum. Genetic alterations are often associated with progression from premalignant lesion (adenoma) to invasive adenocarcinoma. Risk factors for cancer of the colon and rectum include colon polyps, long-standing ulcerative colitis, and genetic family history.

None

The gene represented in this entry may be involved in disease pathogenesis.

Pathway

Energy metabolism; oxidative phosphorylation.

Sequence Similarities

Belongs to the heme-copper respiratory oxidase family.

Cellular localization

Alternative names

COI, COXI, MTCO1, MT-CO1, Cytochrome c oxidase subunit 1, Cytochrome c oxidase polypeptide I

swissprot:P00395 entrezGene:4512 omim:516030