MUC5AC
GeneName
MUC5AC
Summary
MUC5AC, also known as TBM or gastric mucin, is a high molecular weight glycoprotein with a mass of 586 kDa. It is primarily secreted into the mucus layer of various epithelial tissues, particularly in the respiratory and gastrointestinal tracts. MUC5AC plays a crucial role in forming the protective mucus barrier, contributing to the structure of the extracellular matrix and facilitating the binding of metal ions. It is localised in the extracellular space, including the plasma membrane and Golgi lumen, and is involved in maintaining mucosal hydration and protecting epithelial surfaces from pathogens and irritants.
Importance
MUC5AC is relevant to: - Mucosal immunity and host defence due to its role in the formation of mucus barriers that trap pathogens - Respiratory diseases such as asthma and chronic obstructive pulmonary disease (COPD), where abnormal mucus production is a hallmark - Gastrointestinal disorders, including gastric ulcers and cancers, as it is a major component of gastric mucus - Biomarker studies in various diseases, as altered expression levels of MUC5AC can indicate pathological conditions.
Top Products
For researchers investigating MUC5AC, we highly recommend the top-selling recombinant antibody, Anti-Mucin 5AC antibody [45M1] (ab3649). This well-cited product has garnered 178 citations, reflecting its strong reputation in the field. It has been validated for use in immunohistochemistry (IHC) and immunocytochemistry (ICC), making it an excellent choice for those requiring reliable detection of MUC5AC in various sample types. The recombinant nature of this antibody ensures batch-to-batch consistency, providing researchers with confidence in their results. The Anti-Mucin 5AC antibody ELISA Kit (ab77576), supported by 9 citations, is an excellent option for researchers looking to accurately measure MUC5AC levels in their samples.
Abcam Product Citation Summary
The data indicates that MUC5AC is being studied in various contexts related to airway and nasal epithelial cells, particularly in relation to IL-19 regulation and differentiation stages in airway cultures. The use of both human and mouse models highlights the relevance of MUC5AC in respiratory health and disease.
Abcam Product Citation Table
Developmental stage
In airway epithelial cells, expression increases significantly during cell differentiation (at protein level).
Domain
The cysteine residues in the Cys-rich subdomain repeats are not involved in disulfide bonding.
The CTCK domain mediates interchain disulfide bonds with another molecule of MUC5AC.
Function
Gel-forming glycoprotein of gastric and respiratory tract epithelia that protects the mucosa from infection and chemical damage by binding to inhaled microorganisms and particles that are subsequently removed by the mucociliary system (PubMed:14535999, PubMed:14718370). Interacts with H.pylori in the gastric epithelium, Barrett's esophagus as well as in gastric metaplasia of the duodenum (GMD) (PubMed:14535999).
Post-translational modifications
C-, O- and N-glycosylated (PubMed:14718370). O-glycosylated on the second and last Thr of the Thr-/Ser-rich tandem repeats TTPSPVPTTSTTSA (PubMed:14718370, PubMed:22186971, PubMed:25939779). One form of glycosylation is also known as Lewis B (LeB) blood group antigen, a tetrasaccharide consisting of N-acetylglucosamine having a fucosyl residue attached (PubMed:14535999). It has a role as an epitope and antigen and functions as a receptor for H.pylori binding and facilitates infection (PubMed:14535999). C-mannosylation in the Cys-rich subdomains may be required for proper folding of these regions and for export from the endoplasmic reticulum during biosynthesis (PubMed:14718370).
Proteolytic cleavage in the C-terminal is initiated early in the secretory pathway and does not involve a serine protease. The extent of cleavage is increased in the acidic parts of the secretory pathway. Cleavage generates a reactive group which could link the protein to a primary amide.
Tissue Specificity
Highly expressed in surface mucosal cells of respiratory tract and stomach epithelia. Overexpressed in a number of carcinomas. Also expressed in Barrett's esophagus epithelium and in the proximal duodenum.
Cellular localization
- Secreted
Alternative names
MUC5, MUC5AC, Mucin-5AC, MUC-5AC, Gastric mucin, Major airway glycoprotein, Tracheobronchial mucin, TBM