MYOG
GeneName
MYOG
Summary
MYOG, also known as myogenic factor or myogenin, is a 25kDa transcription factor that plays a pivotal role in muscle development and differentiation. It is primarily expressed in skeletal muscle tissues and is localised within the nucleus, where it associates with chromatin and forms protein-DNA complexes to regulate gene expression. MYOG is essential for myoblast differentiation and the commitment of muscle cell fate, engaging in various molecular functions such as sequence-specific DNA binding and E-box binding, which are critical for activating muscle-specific genes during myogenesis.
Importance
MYOG is relevant to: - Muscle regeneration and repair processes, as it regulates myoblast differentiation and fusion during muscle development - Understanding muscle-related diseases, including muscular dystrophies, due to its role in skeletal muscle fibre development - The effects of hormonal and growth factor signalling on muscle adaptation, particularly in response to stimuli like estradiol and tumour necrosis factor - Research into muscle atrophy and related conditions, since it is involved in the positive regulation of myotube differentiation and the negative regulation of cell population proliferation
Top Products
For researchers investigating MYOG, we recommend two excellent primary antibodies. The first is the well-cited Anti-Myogenin antibody [F5D] (ab1835), which has garnered 112 citations, reflecting its strong reputation in the field. This monoclonal antibody is highly effective for Western blotting (WB), immunocytochemistry (ICC), and immunohistochemistry (IHC). Additionally, we offer the recombinant antibody, Anti-Myogenin antibody [EPR4789] (ab124800). This product has also been validated for WB, ICC, and IHC, making it a versatile option for those seeking the consistency and reliability that recombinant antibodies provide. With 68 citations, it is gaining traction among researchers studying MYOG.
Abcam Product Citation Summary
The data indicates that MYOG is frequently studied in the context of muscle differentiation and regeneration, particularly in mouse models. Various studies utilise both Western blotting and immunocytochemistry to investigate the role of MYOG in myoblast and myotube differentiation, as well as the effects of specific microRNAs on muscle development. Additionally, research involving domestic pigs highlights the relevance of MYOG in satellite cell differentiation.
Abcam Product Citation Table
Function
Acts as a transcriptional activator that promotes transcription of muscle-specific target genes and plays a role in muscle differentiation, cell cycle exit and muscle atrophy. Essential for the development of functional embryonic skeletal fiber muscle differentiation. However is dispensable for postnatal skeletal muscle growth; phosphorylation by CAMK2G inhibits its transcriptional activity in respons to muscle activity. Required for the recruitment of the FACT complex to muscle-specific promoter regions, thus promoting gene expression initiation. During terminal myoblast differentiation, plays a role as a strong activator of transcription at loci with an open chromatin structure previously initiated by MYOD1. Together with MYF5 and MYOD1, co-occupies muscle-specific gene promoter core regions during myogenesis. Cooperates also with myocyte-specific enhancer factor MEF2D and BRG1-dependent recruitment of SWI/SNF chromatin-remodeling enzymes to alter chromatin structure at myogenic late gene promoters. Facilitates cell cycle exit during terminal muscle differentiation through the up-regulation of miR-20a expression, which in turn represses genes involved in cell cycle progression. Binds to the E-box containing (E1) promoter region of the miR-20a gene. Plays also a role in preventing reversal of muscle cell differentiation. Contributes to the atrophy-related gene expression in adult denervated muscles. Induces fibroblasts to differentiate into myoblasts (By similarity).
Post-translational modifications
Phosphorylated by CAMK2G on threonine and serine amino acids in a muscle activity-dependent manner. Phosphorylation of Thr-87 impairs both DNA-binding and trans-activation functions in contracting muscles (By similarity).
Cellular localization
- Nucleus
- Recruited to late myogenic gene promoter regulatory sequences with SMARCA4/BRG1/BAF190A and SWI/SNF chromatin-remodeling enzymes to promote chromatin-remodeling and transcription initiation in developing embryos.
Alternative names
BHLHC3, MYF4, MYOG, Myogenin, Class C basic helix-loop-helix protein 3, Myogenic factor 4, bHLHc3, Myf-4