NAP1L4
Function
Acts as a histone chaperone in nucleosome assembly.
Post-translational modifications
Phosphorylated at the G0/G1 boundary but it is not phosphorylated in S-phase. Phosphorylated protein remains in the cytoplasm in a complex with histones during the G0/G1 transition, whereas dephosphorylation triggers its transport into the nucleus at the G1/S-boundary.
Polyglutamylated by TTLL4, a modification that occurs exclusively on glutamate residues and results in polyglutamate chains on the gamma-carboxyl group. Some residues may also be monoglycylated but not polyglycylated due to the absence of functional TTLL10 in human (By similarity).
Sequence Similarities
Belongs to the nucleosome assembly protein (NAP) family.
Tissue Specificity
Ubiquitous. Biallelically expressed in fetal and adult tissues. Highest levels in testis.
Cellular localization
- Nucleus
- Cytoplasm
- Present in the cytoplasm and excluded from the nucleus during G0/G1 phase, then relocates to the nucleus by the time cells are in S phase (PubMed:9325046). Phosphorylated form localizes in the cytoplasm during the G0/G1 transition, whereas dephosphorylation leads to relocalization into the nucleus at the G1/S-boundary (PubMed:10764593).
Alternative names
NAP2, NAP1L4, Nucleosome assembly protein 1-like 4, Nucleosome assembly protein 2, NAP-2