Ncf1
Domain
The PX domain mediates interaction with phosphatidylinositol 3,4-bisphosphate and other anionic phospholipids. In the autoinhibited, unphosphorylated state an intramolecular interaction with the C-terminal SH3 domain precludes phospholipid binding and interaction with CYBA. Phosphorylation disrupts the autoinhibited state (By similarity).
Function
NCF2, NCF1, and a membrane bound cytochrome b558 are required for activation of the latent NADPH oxidase (necessary for superoxide production).
Post-translational modifications
Phosphorylated by PRKCD; phosphorylation induces activation of NCF1 and NADPH oxidase activity.
Cellular localization
- Cytoplasm
- Cytosol
- Membrane
- Peripheral membrane protein
- Cytoplasmic side
Alternative names
Neutrophil cytosol factor 1, NCF-1, 47 kDa neutrophil oxidase factor, NCF-47K, Neutrophil NADPH oxidase factor 1, p47-phox, Ncf1