NEFM phospho S614 + S619

Function

Neurofilaments usually contain three intermediate filament proteins: NEFL, NEFM, and NEFH which are involved in the maintenance of neuronal caliber. May additionally cooperate with the neuronal intermediate filament proteins PRPH and INA to form neuronal filamentous networks (By similarity).

Post-translational modifications

There are a number of repeats of the tripeptide K-S-P, NFM is phosphorylated on a number of the serines in this motif. It is thought that phosphorylation of NFM results in the formation of interfilament cross bridges that are important in the maintenance of axonal caliber.

Phosphorylation seems to play a major role in the functioning of the larger neurofilament polypeptides (NF-M and NF-H), the levels of phosphorylation being altered developmentally and coincidentally with a change in the neurofilament function.

Phosphorylated in the head and rod regions by the PKC kinase PKN1, leading to the inhibition of polymerization.

Sequence similarities

Belongs to the intermediate filament family.

Cellular localization

• Cytoplasm
• Cytoskeleton
• Cell projection
• Axon

Alternative names

• Neurofilament medium polypeptide
• NF-M
• 160 kDa neurofilament protein
• Neurofilament 3
• Neurofilament triplet M protein
• NFM
• NEFM
• NEF3

Database links

• swissprot:P07197

Target type

Proteins

Primary research area

Neuroscience

Molecular weight

102472Da