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NEK7

OverviewProducts

Domain

Displays an autoinhibited conformation: Tyr-97 side chain points into the active site, interacts with the activation loop, and blocks the alphaC helix. The autoinhibitory conformation is released upon binding with NEK9.

The NTE (N-terminal extension) motif is a structural component of the catalytic domain and thus contributes to activity.

Function

Protein kinase which plays an important role in mitotic cell cycle progression (PubMed:17101132). Required for microtubule nucleation activity of the centrosome, robust mitotic spindle formation and cytokinesis (PubMed:17586473, PubMed:19414596). Phosphorylates RPS6KB1 (By similarity). Phosphorylates EML4 at 'Ser-146', promoting its dissociation from microtubules during mitosis which is required for efficient chromosome congression (PubMed:31409757).

Post-translational modifications

Phosphorylation at Ser-195 required for its activation.

Sequence similarities

Belongs to the protein kinase superfamily. NEK Ser/Thr protein kinase family. NIMA subfamily.

Tissue specificity

Highly expressed in lung, muscle, testis, brain, heart, liver, leukocyte and spleen. Lower expression in ovary, prostate and kidney. No expression seen in small intestine.

Cellular localization

  • Nucleus
  • Cytoplasm
  • Cytoplasm
  • Cytoskeleton
  • Spindle pole
  • Cytoplasm
  • Cytoskeleton
  • Microtubule organizing center
  • Centrosome
  • Present at centrosome throughout the cell cycle (PubMed:17586473). Also detected at spindle midzone of the anaphase cells and eventually concentrates at the midbody (PubMed:17586473). Interaction with ANKS3 prevents its translocation to the nucleus (By similarity).

Alternative names

  • Serine/threonine-protein kinase Nek7
  • Never in mitosis A-related kinase 7
  • NimA-related protein kinase 7
  • NEK7

Database links

Target type

Proteins

Molecular weight

34551Da