NES
GeneName
NES
Summary
Nestin, also known as LPCAT, is a 177 kDa intermediate filament protein that is primarily expressed in neural stem cells and during neurogenesis. It is localised to the cytoplasm and is a key component of the intermediate filament cytoskeleton. Nestin plays a crucial role in brain development and the morphogenesis of cell projections, contributing to the structural integrity and function of neural cells. Additionally, it is involved in various cellular processes, including the G2/M transition of the mitotic cell cycle and the regulation of apoptotic processes in neurons. Nestin is also associated with the proliferation of neural precursor cells and stem cells, highlighting its importance in developmental biology and tissue regeneration.
Importance
Nestin is relevant to: - The study of neural development and neurogenesis due to its expression in neural stem cells and role in brain formation - Regenerative medicine and stem cell research as it serves as a marker for neural progenitor cells - Understanding neurodegenerative diseases, where alterations in Nestin expression may impact neuronal survival and plasticity - Cancer research, particularly in gliomas, where Nestin expression is associated with tumour aggressiveness and stem-like properties
Top Products
For researchers investigating the NES gene, we recommend two excellent primary antibodies. The first is the well-cited Anti-Nestin antibody [10C2] (ab22035), a trusted choice for immunocytochemistry (ICC) and western blotting (WB), with 194 citations highlighting its reliability in the field. Additionally, we offer the recombinant Anti-Nestin antibody [SP103] (ab105389), which has been validated in knockout models and is suitable for a broader range of applications, including ICC, immunohistochemistry (IHC), WB, and flow cytometry (FC). With 102 citations, this recombinant antibody is an outstanding option for researchers seeking consistency and versatility in their experiments. The Anti-Nestin antibody [SP103] - Neural Stem Cell Marker ELISA Kit (ab105389), with 102 citations, is an excellent option for researchers looking to accurately measure Nestin levels in their studies.
Abcam Product Citation Summary
The data indicates that NES antibodies from Abcam are primarily used in human studies, particularly in the context of stem cell research and differentiation. The applications include immunocytochemistry and immunofluorescence, highlighting the importance of NES in neural crest stem cell differentiation and neural progenitor differentiation. Additionally, there is usage in western blotting for exosomal protein extracts and embryonic stem cells, suggesting a broader relevance in developmental biology and cancer research.
Abcam Product Citation Table
Developmental stage
Upon terminal neural differentiation, nestin is down-regulated and replaced by neurofilaments.
Function
Required for brain and eye development. Promotes the disassembly of phosphorylated vimentin intermediate filaments (IF) during mitosis and may play a role in the trafficking and distribution of IF proteins and other cellular factors to daughter cells during progenitor cell division. Required for survival, renewal and mitogen-stimulated proliferation of neural progenitor cells (By similarity).
Post-translational modifications
Constitutively phosphorylated. This increases during mitosis when the cytoplasmic intermediate filament network is reorganized (By similarity).
Sequence Similarities
Belongs to the intermediate filament family.
Tissue Specificity
CNS stem cells.
Alternative names
Nbla00170, NES, Nestin