NOS3 phospho S1177
Function
Produces nitric oxide (NO) which is implicated in vascular smooth muscle relaxation through a cGMP-mediated signal transduction pathway (PubMed:1378832). NO mediates vascular endothelial growth factor (VEGF)-induced angiogenesis in coronary vessels and promotes blood clotting through the activation of platelets.
Isoform eNOS13C
Lacks eNOS activity, dominant-negative form that may down-regulate eNOS activity by forming heterodimers with isoform 1.
Involvement in disease
Variation Asp-298 in NOS3 may be associated with susceptibility to coronary spasm.
Post-translational modifications
Phosphorylation by AMPK at Ser-1177 in the presence of Ca(2+)-calmodulin (CaM) activates activity. In absence of Ca(2+)-calmodulin, AMPK also phosphorylates Thr-495, resulting in inhibition of activity (By similarity). Phosphorylation of Ser-114 by CDK5 reduces activity.
Sequence Similarities
Belongs to the NOS family.
Tissue Specificity
Platelets, placenta, liver and kidney.
Cellular localization
- Cell membrane
- Membrane
- Caveola
- Cytoplasm
- Cytoskeleton
- Golgi apparatus
- Specifically associates with actin cytoskeleton in the G2 phase of the cell cycle; which is favored by interaction with NOSIP and results in a reduced enzymatic activity.
Alternative names
Nitric oxide synthase 3, Constitutive NOS, EC-NOS, NOS type III, cNOS, NOSIII, Endothelial NOS, eNOS, NOS3
Database links
Other research areas
- Cardiovascular
- Immuno-oncology