JavaScript is disabled in your browser. Please enable JavaScript to view this website.

NR3C2

GeneName

NR3C2

Summary

NR3C2, commonly referred to as the mineralocorticoid receptor (MCR), is a 107 kDa nuclear receptor that functions primarily as a transcription factor. It is expressed in various tissues, including the kidney, heart, and brain, where it plays a pivotal role in mediating the effects of mineralocorticoids such as aldosterone. NR3C2 is involved in the regulation of sodium and potassium homeostasis, blood pressure control, and the modulation of inflammatory responses. The receptor operates through binding to specific DNA sequences in target genes, influencing transcriptional activity and thereby impacting various physiological processes. Its activity is linked to several cellular components, including chromatin, the nucleus, and the endoplasmic reticulum membrane, reflecting its role in gene regulation and signal transduction pathways.

Importance

NR3C2 is relevant to: - Hypertension and cardiovascular diseases due to its role in sodium retention and blood pressure regulation - Metabolic disorders as it influences electrolyte balance and fluid homeostasis - Inflammatory responses and conditions, given its involvement in non-canonical NF-kappaB signalling - Potential therapeutic targets for diseases related to mineralocorticoid excess or deficiency, highlighting its significance in endocrine research

Top Products

For researchers investigating NR3C2, we highly recommend the well-cited Anti-Mineralocorticoid Receptor antibody [H10E4C9F] (ab2774). This monoclonal antibody has garnered 34 citations, reflecting its reliability and trust within the scientific community. It is particularly effective for immunohistochemistry (IHC), immunocytochemistry (ICC), and flow cytometry (FC), making it a versatile tool for various experimental needs. This antibody is an excellent choice for those looking to achieve consistent and reproducible results in their research on NR3C2.

Abcam Product Citation Summary

The NR3C2 gene, which encodes the mineralocorticoid receptor, has been studied in various contexts including kidney, heart, and cancer tissues. The use of Abcam antibody ab2774 in Western blotting and immunohistochemistry highlights its relevance in understanding receptor expression and activation in both normal and pathological states.

Abcam Product Citation Table

Product Code
Species
Application
Study Context
PMID
ab2774
Rat
WB
Mineralocorticoid receptor expression in kidneys
30819180
ab2774
Ovis aries
WB
29267325
ab2774
Human
IHC
Colonic mucosa and adenocarcinoma
23555666
ab2774
Human
WB, IHC
Mineralocorticoid receptor activation in HCT116 cells
23555666
ab2774
Rattus norvegicus
WB
Effects of maternal pentaerythritol tetranitrate treatment in offspring kidneys
32410988
ab2774
Mouse
WB
Adrenal tissue
25784875
ab2774
Human
WB
Glioblastoma samples and regulation by miR-1204
32280303

Domain

Composed of three domains: a modulating N-terminal domain, a DNA-binding domain and a C-terminal ligand-binding domain.

Function

Receptor for both mineralocorticoids (MC) such as aldosterone and glucocorticoids (GC) such as corticosterone or cortisol. Binds to mineralocorticoid response elements (MRE) and transactivates target genes. The effect of MC is to increase ion and water transport and thus raise extracellular fluid volume and blood pressure and lower potassium levels.

Involvement in disease

Pseudohypoaldosteronism 1, autosomal dominant

PHA1A

A salt wasting disease resulting from target organ unresponsiveness to mineralocorticoids. PHA1A is a mild form characterized by target organ defects confined to kidney. Patients may present with neonatal renal salt wasting with hyperkalaemic acidosis despite high aldosterone levels. These patients improve with age and usually become asymptomatic without treatment.

None

The disease is caused by variants affecting the gene represented in this entry.

Early-onset hypertension with severe exacerbation in pregnancy

EOHSEP

Inheritance is autosomal dominant. The disease is characterized by the onset of severe hypertension before the age of 20, and by suppression of aldosterone secretion.

None

The disease is caused by variants affecting the gene represented in this entry.

Post-translational modifications

Phosphorylated.

Sequence Similarities

Belongs to the nuclear hormone receptor family. NR3 subfamily.

Tissue Specificity

Ubiquitous. Highly expressed in distal tubules, convoluted tubules and cortical collecting duct in kidney, and in sweat glands. Detected at lower levels in cardiomyocytes, in epidermis and in colon enterocytes.

Cellular localization

Alternative names

MCR, MLR, NR3C2, Mineralocorticoid receptor, MR, Nuclear receptor subfamily 3 group C member 2

swissprot:P08235 entrezGene:4306 omim:600983

Other research areas