NT5E
GeneName
NT5E
Summary
NT5E, also known as CD73 or NTE, is a 63 kDa glycoprotein predominantly expressed on the surface of various cell types, including endothelial cells and immune cells. It is localised to the plasma membrane and plays a critical role in the hydrolysis of extracellular nucleotides, converting AMP to adenosine. This enzymatic activity is vital for adenosine biosynthesis, which influences numerous biological processes such as inflammation and immune responses. NT5E is also involved in calcium ion homeostasis and has been implicated in leukocyte adhesion and the regulation of inflammatory responses. Its presence in extracellular exosomes suggests a role in intercellular communication.
Importance
NT5E is relevant to: - The modulation of immune responses through its production of adenosine, which can inhibit T cell activation and promote immune tolerance - The regulation of inflammation, as it contributes to the negative regulation of inflammatory responses - Cancer biology, where its expression may affect tumour progression and the tumour microenvironment - Cardiovascular health, due to its involvement in ATP metabolism and vascular function - Bone metabolism, given its role in the inhibition of non-skeletal tissue mineralization
Top Products
For researchers investigating NT5E, we recommend two excellent primary antibodies that cater to different experimental needs. The first is the well-cited monoclonal antibody, Anti-CD73 antibody [7G2] (ab54217), which has garnered 30 citations and is particularly effective for flow cytometry (FC) and immunocytochemistry (ICC). This antibody is a trusted choice for many in the field. Additionally, we offer the recombinant monoclonal antibody, Anti-CD73 antibody [EPR6114] (ab133582). This product stands out with 34 citations and has been validated in knockout models, making it suitable for a variety of applications, including immunohistochemistry (IHC) and western blotting (WB). The recombinant nature of this antibody ensures batch-to-batch consistency, providing researchers with reliable results in their studies of NT5E. The Human CD73 ELISA Kit (ab254513) is an excellent option for researchers looking to measure NT5E levels in their samples.
Abcam Product Citation Summary
The data indicates that NT5E is being investigated in the context of human ovarian tumors, specifically focusing on cancer survival analysis. The use of IHC-IF suggests a detailed examination of protein expression in these tumour samples.
Abcam Product Citation Table
Function
Catalyzes the hydrolysis of nucleotide monophosphates, releasing inorganic phosphate and the corresponding nucleoside, with AMP being the preferred substrate (PubMed:21933152, PubMed:22997138, PubMed:23142347, PubMed:24887587, PubMed:34403084). Shows a preference for ribonucleotide monophosphates over their equivalent deoxyribose forms (PubMed:34403084). Other substrates include IMP, UMP, GMP, CMP, dAMP, dCMP, dTMP, NAD and NMN (PubMed:21933152, PubMed:22997138, PubMed:23142347, PubMed:24887587, PubMed:34403084).
Involvement in disease
Calcification of joints and arteries
CALJA
A condition characterized by adult-onset calcification of the lower extremity arteries, including the iliac, femoral and tibial arteries, and hand and foot capsule joints. Age of onset has been reported as early as the second decade of life, usually involving intense joint pain or calcification in the hands.
None
The disease is caused by variants affecting the gene represented in this entry.
Sequence Similarities
Belongs to the 5'-nucleotidase family.
Cellular localization
- Cell membrane
- Lipid-anchor
- GPI-anchor
Alternative names
CD73, NT5, NTE, NT5E, 5'-nucleotidase, 5'-NT, 5'-deoxynucleotidase, Ecto-5'-nucleotidase, IMP-specific 5'-nucleotidase, Thymidylate 5'-phosphatase