Nucleoprotein TPR
GeneName
TPR
Summary
TPR, also known as trypanothione reductase or thiol protease, is a 267 kDa protein that is primarily located in the cytoplasm and nucleus. It plays a critical role in various cellular processes, including cell division and nucleocytoplasmic transport. TPR is involved in chromatin binding and interacts with several complexes, such as the dynein complex and the mitotic spindle. It is essential for mRNA export from the nucleus and regulates transcription and translation processes. Additionally, TPR is implicated in the assembly of the mitotic spindle and the organisation of nuclear pores, contributing to cellular responses to stress and growth factors.
Importance
TPR is relevant to: - Understanding the mechanisms of cell division and mitotic regulation, which are crucial for cancer research - Investigating the role of nuclear transport in cellular function and disease - Exploring the impact of heat shock responses on protein stability and function - Studying the dynamics of chromatin and its regulation, which has implications in gene expression and epigenetics
Top Products
For researchers investigating TPR, we recommend two excellent primary antibodies that cater to different applications. The first is the well-cited polyclonal antibody, Anti-TPR antibody (ab84516), which has garnered 21 citations and is particularly effective for immunocytochemistry (ICC) and immunohistochemistry (IHC). This antibody is a trusted choice for those focusing on these specific techniques. Additionally, we offer the recombinant antibody, Anti-TPR antibody [EPR8982] (ab170940), which is suitable for Western blotting (WB) and ICC. With 4 citations, this recombinant product provides the added benefit of batch-to-batch consistency, making it an excellent option for researchers seeking reliable results in their studies of TPR.
Abcam Product Citation Summary
The TPR target has been studied in human HeLa cells using western blotting techniques. Both antibodies, ab170940 and ab70610, were employed in the same study, indicating a focus on the detection of TPR in this specific cell line.
Abcam Product Citation Table
Domain
The N-terminal domain mediates intranuclear attachment to the nuclear pore complex. The C-terminal domain mediates its nuclear import.
Function
Component of the nuclear pore complex (NPC), a complex required for the trafficking across the nuclear envelope. Functions as a scaffolding element in the nuclear phase of the NPC essential for normal nucleocytoplasmic transport of proteins and mRNAs, plays a role in the establishment of nuclear-peripheral chromatin compartmentalization in interphase, and in the mitotic spindle checkpoint signaling during mitosis. Involved in the quality control and retention of unspliced mRNAs in the nucleus; in association with NUP153, regulates the nuclear export of unspliced mRNA species bearing constitutive transport element (CTE) in a NXF1- and KHDRBS1-independent manner. Negatively regulates both the association of CTE-containing mRNA with large polyribosomes and translation initiation. Does not play any role in Rev response element (RRE)-mediated export of unspliced mRNAs. Implicated in nuclear export of mRNAs transcribed from heat shock gene promoters; associates both with chromatin in the HSP70 promoter and with mRNAs transcribed from this promoter under stress-induced conditions. Modulates the nucleocytoplasmic transport of activated MAPK1/ERK2 and huntingtin/HTT and may serve as a docking site for the XPO1/CRM1-mediated nuclear export complex. According to some authors, plays a limited role in the regulation of nuclear protein export (PubMed:11952838, PubMed:22253824). Also plays a role as a structural and functional element of the perinuclear chromatin distribution; involved in the formation and/or maintenance of NPC-associated perinuclear heterochromatin exclusion zones (HEZs). Finally, acts as a spatial regulator of the spindle-assembly checkpoint (SAC) response ensuring a timely and effective recruitment of spindle checkpoint proteins like MAD1L1 and MAD2L1 to unattached kinetochore during the metaphase-anaphase transition before chromosome congression. Its N-terminus is involved in activation of oncogenic kinases.
Involvement in disease
A chromosomal aberration involving TPR has been found in papillary thyroid carcinomas (PTCs). Intrachromosomal rearrangement that links the 5'-end of the TPR gene to the protein kinase domain of NTRK1 forms the fusion protein TRK-T1. TRK-T1 is a 55 kDa protein reacting with antibodies against the carboxy terminus of the NTRK1 protein.
Involved in tumorigenic rearrangements with the MET.
Intellectual developmental disorder, autosomal recessive 79
MRT79
An autosomal recessive neurodevelopmental disorder apparent from infancy and characterized by global developmental delay, severe intellectual disability, poor or absent speech, ataxia, and postnatal microcephaly.
None
The disease is caused by variants affecting the gene represented in this entry.
Post-translational modifications
Phosphorylated. Phosphorylation occurs on serine and threonine residues (comprised in the C-terminal region) by MAPK1/ERK2 and stabilizes the interaction between these two proteins.
Proteolytically degraded after poliovirus (PV) infection; degradation is restricted to its unfolded C-terminal tail domain whereas its coiled-coil domain containing NCP- and NUP153-binding domains withstand degradation.
Sequence Similarities
Belongs to the TPR family.
Tissue Specificity
Expressed in esophagus, ovary, liver, skin, smooth muscles, cerebrum and fetal cerebellum (at protein level). Highest in testis, lung, thymus, spleen and brain, lower levels in heart, liver and kidney.
Cellular localization
- Nucleus
- Nucleus membrane
- Peripheral membrane protein
- Nucleoplasmic side
- Nucleus envelope
- Nucleus
- Nuclear pore complex
- Cytoplasm
- Cytoplasm
- Cytoskeleton
- Spindle
- Chromosome
- Centromere
- Kinetochore
- Nucleus membrane
- Peripheral membrane protein
- Cytoplasmic side
- Detected as discrete intranuclear foci with IFI204 (By similarity). In interphase, localizes to the nucleoplasmic side of the nuclear pore complex (NPC) core structure, forming a fibrous structure called the nuclear basket (PubMed:34440706). Detected exclusively to the cytoplasmic margin of NPC (PubMed:7798308). Docking to the inner nucleoplasmic side of the NPC is mediated through binding to nucleoporins. Anchored by NUP153 to the NPC. The assembly of the NPC is a stepwise process in which Trp-containing peripheral structures assemble after other components, including p62. Detected as filaments that emanate from the nuclear basket of the NPC and extend to the nucleolus to delineate a chromatin-free network extending from the nuclear envelope to the perinucleolar region. Detected in diffuse and discrete spheroidal intranuclear foci. Nucleocytoplasmic shuttling protein imported into the nucleus in a XPO1/CRM1- and Importin alpha/Importin beta receptor-dependent manner. Remains localized to the nuclear membrane after poliovirus (PV) infection. During mitosis, remains associated with the nuclear envelope until prometaphase. Associated with the mitotic spindle from late prometaphase until anaphase. Reorganized during mitosis in a viscous and dynamic nuclear-derived spindle matrix that embeds the microtubule spindle apparatus from pole to pole in a microtubule-independent manner. Recruited to the reforming nuclear envelope during telophase and cytokinesis. Detected at kinetochores during prometaphase (PubMed:18981471). Colocalizes with MAD2L1 in the spindle matrix but not at kinetochore (PubMed:19273613). Colocalizes with dynein, dynactin, tubulin at kinetochore during the metaphase-anaphase transition. Colocalizes with DYNLL1 at the mitotic spindle.
Alternative names
Nucleoprotein TPR, Megator, NPC-associated intranuclear protein, Translocated promoter region protein, TPR