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NUDT5

Function

Enzyme that can either act as an ADP-sugar pyrophosphatase in absence of diphosphate or catalyze the synthesis of ATP in presence of diphosphate (PubMed:27257257). In absence of diphosphate, hydrolyzes with similar activities various modified nucleoside diphosphates such as ADP-ribose, ADP-mannose, ADP-glucose, 8-oxo-GDP and 8-oxo-dGDP (PubMed:10567213, PubMed:10722730, PubMed:19699693, PubMed:21389046, PubMed:17052728). Can also hydrolyze other nucleotide sugars with low activity (PubMed:19699693, PubMed:21389046). In presence of diphosphate, mediates the synthesis of ATP in the nucleus by catalyzing the conversion of ADP-ribose to ATP and ribose 5-phosphate. Nuclear ATP synthesis takes place when dephosphorylated at Thr-45 (PubMed:27257257). Nuclear ATP generation is required for extensive chromatin remodeling events that are energy-consuming (PubMed:27257257). Does not play a role in U8 snoRNA decapping activity (By similarity). Binds U8 snoRNA (By similarity).

Post-translational modifications

Phosphorylation at Thr-45 is required for homodimer stability; dephosphorylation results in destabilization of the homodimer. Dephosphorylation at Thr-45 promotes the ATP-synthesis activity.

Sequence similarities

Belongs to the Nudix hydrolase family.

Tissue specificity

Widely expressed. Most abundant in liver.

Cellular localization

  • Nucleus

Alternative names

  • ADP-sugar pyrophosphatase
  • 8-oxo-dGDP phosphatase
  • Nuclear ATP-synthesis protein NUDIX5
  • Nucleoside diphosphate-linked moiety X motif 5
  • YSA1H
  • Nudix motif 5
  • hNUDT5
  • NUDT5
  • HSPC115
  • NUDIX5

Target type

Proteins

Molecular weight

24328Da