PHOSPHO1
Function
Phosphatase that has a high activity toward phosphoethanolamine (PEA) and phosphocholine (PCho) (PubMed:15175005). Involved in the generation of inorganic phosphate for bone mineralization (By similarity). Acts in a non-redundant manner with PHOSPHO1 in skeletal mineralization: while PHOSPHO1 mediates the initiation of hydroxyapatite crystallization in the matrix vesicles (MVs), ALPL/TNAP catalyzes the spread of hydroxyapatite crystallization in the extracellular matrix (By similarity).
Sequence Similarities
Belongs to the HAD-like hydrolase superfamily. PHOSPHO family.
Tissue Specificity
Expressed at sites of mineralization in bone and cartilage. Highly expressed in osteoblast cell line SaOS-2 which produces a mineralized matrix, but not in MG-63 cell line, which do not mineralize.
Cellular localization
- Extracellular vesicle
- Localizes to special class of extracellular vesicles, named matrix vesicles (MVs), which are released by osteogenic cells.
Alternative names
Phosphoethanolamine/phosphocholine phosphatase, PHOSPHO1