PIAS3
Domain
The PINIT domain of PIAS3 is required for STAT3-PIAS3 interaction and for translocation to the nucleus.
The LXXLL motif is a transcriptional coregulator signature.
Function
Functions as an E3-type small ubiquitin-like modifier (SUMO) ligase, stabilizing the interaction between UBE2I and the substrate, and as a SUMO-tethering factor. Plays a crucial role as a transcriptional coregulation in various cellular pathways, including the STAT pathway and the steroid hormone signaling pathway. Involved in regulating STAT3 signaling via inhibiting STAT3 DNA-binding and suppressing cell growth. Enhances the sumoylation of MTA1 and may participate in its paralog-selective sumoylation (PubMed:21965678, PubMed:9388184). Sumoylates CCAR2 which promotes its interaction with SIRT1 (PubMed:25406032). Diminishes the sumoylation of ZFHX3 by preventing the colocalization of ZFHX3 with SUMO1 in the nucleus (PubMed:24651376).
Pathway
Protein modification; protein sumoylation.
Post-translational modifications
Sumoylated.
Sequence Similarities
Belongs to the PIAS family.
Tissue Specificity
Widely expressed.
Cellular localization
- Cytoplasm
- Nucleus
- Nucleus speckle
- Colocalizes with MITF in the nucleus. Colocalizes with GFI1 in nuclear dots. Colocalizes with SUMO1 in nuclear granules.
Alternative names
E3 SUMO-protein ligase PIAS3, E3 SUMO-protein transferase PIAS3, Protein inhibitor of activated STAT protein 3, PIAS3