PKMYT1
Domain
The membrane-association motif is essential for the localization to membrane of Golgi stack (PubMed:10504341). According to some authors, it is a transmembrane domain; the existence of a transmembrane region is however unproven (PubMed:10504341).
Function
Acts as a negative regulator of entry into mitosis (G2 to M transition) by phosphorylation of the CDK1 kinase specifically when CDK1 is complexed to cyclins (PubMed:10373560, PubMed:10504341, PubMed:9001210, PubMed:9268380). Mediates phosphorylation of CDK1 predominantly on 'Thr-14'. Also involved in Golgi fragmentation (PubMed:9001210, PubMed:9268380). May be involved in phosphorylation of CDK1 on 'Tyr-15' to a lesser degree, however tyrosine kinase activity is unclear and may be indirect (PubMed:9001210, PubMed:9268380).
Post-translational modifications
Autophosphorylated. Phosphorylated by CDC2-CCNB1 complexes on undefined serine and threonine residues. The phosphorylation by CDC2-CCNB1 complexes may inhibit the catalytic activity.
Sequence Similarities
Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. WEE1 subfamily.
Cellular localization
- Endoplasmic reticulum membrane
- Peripheral membrane protein
- Golgi apparatus membrane
- Peripheral membrane protein
Alternative names
MYT1, PKMYT1, Membrane-associated tyrosine- and threonine-specific cdc2-inhibitory kinase, Myt1 kinase